Résumé
Background: in this study, using a modified microtitre plate and scanning electron microscope, we investigated biofilm production by Pseudomonas aeruginosa .biofilm productions are difficult to eradicate with antimicrobial treatment and up to 60% of all human infections are caused by biofilms. Bacterial biofilms may be found at various sites, including mucus membrane, teeth and indwelling medical devices. Pseudomonas aeruginosa is one of the most important opportunistic bacteria and producing biofilm. It has been reported that biofilm forming bacteria are more resistance to antibiotic treatment and immunologic attack
Materials and methods: clinical isolates of P.aeruginosa were collected from hospital and identified by biochemical tests. A modified microtitre plate as described by stepanovic was used to determine the biofilm formation capacity of P.aeruginosa and measured using ELISA reader. The bacteria biofilm on the Teflon sheets and cather were fixed by modified method described by Ishila. They were observed with scanning electron microscope
Results: the results demonstrated that Pseudornonas aeruginosa strain M214 denser biofilm formation compared with other strain, with OD=2.89 using modified microtitre plate test. Scanning electron micrograph of bacteria on the Teflon and catherter that had been incubated with P.aeruginosa M214 for 1 and 7 days were investigated. The results showed biofilm formation on catherter was denser that Teflon sheets. The bacteria incubated for 7 days on Teflon and catherter were covered with thick membranous and fibrous structure, unlike bacteria incubated for 1 day
Conclusion: It seems modified microtitre plate is better and easier way to use in diagnostic labratories regard to treatment difficulteis, it strongly recommend to study the biofilm before antibiotic adminstration
Résumé
Background: in this study, using a modified microtitre plate and scanning electron microscope, we investigated biofilm production by Pseudomonas aeruginosa .biofilm productions are difficult to eradicate with antimicrobial treatment and up to 60% of all human infections are caused by biofilms. Bacterial biofilms may be found at various sites, including mucus membrane, teeth and indwelling medical devices. Pseudomonas aeruginosa is one of the most important opportunistic bacteria and producing biofilm. It has been reported that biofilm forming bacteria are more resistance to antibiotic treatment and immunologic attack
Materials and Methods: clinical isolates of P. aeruginosa were collected from hospital and identified by biochemical tests. A modified microtitre plate as described by stepanovic was used to determine the biofilm formation capacity of P. aeruginosa and measured using ELISA reader. The bacteria biofilm on the Teflon sheets and cather were fixed by modified method described by Ishila. They were observed with scanning electron microscope
Results: the results demonstrated that Pseudomonas aeruginosa strain M214 denser biofilm formation compared with other strain, with 00=2.89 using modified microtitre plate test. Scanning electron micrograph of bacteria on the Teflon and catherter that had been incubated with P. aeruginosa M214 for 1 and 7 days were investigated. The results showed biofilm formation on catherter was denser that Teflon sheets. The bacteria incubated for 7 days on Teflon and catherter were covered with thick membranous and fibrous structure, unlike bacteria incubated for 1 day
Conclusion: It seems modified microtitre plate is better and easier way to use in diagnostic labratories regard to treatment difficulties, it strongly recommend to study the biofilm before antibiotic adminstration