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Objective To analyze the expressions of osteopontin (OPN)and chemokine (CXC-subfamily)receptor 4 (CXCR4)in different ovarian tissues,and to explore the role of OPN and CXCR4 in occurrence,development and metastasis of human ovarian cancer.Methods The expressions of OPN and CXCR4 in normal ovarian tissue (n=20),benign ovarian epithelial tumor (n=20)and epithelial ovarian cancers tissues (n=40)were detected by immunohistochemical SP method,and the expression rates of OPN and CXCR4 in epithelial ovarian cancer tissue with different clinical characteristics were analyzed.Results The positive expressions rate of OPN in epithelial ovarian cancer tissue was significantly higher than those in normal ovarian tissue and benign ovarian tumor tissue, and there were significant differences in the positive expression rates of OPN between different pathological stages (G1 ,G2 ,G3 )of epithelial ovarian cancer (P 0.05).There was no positive expression of CXCR4 protein in normal ovarian tissue and benign ovarian tumor tissues , but there was positive expression in epithelial ovarian cancer tissue.Besides,the expressions of CXCR4 protein were not significantly different among different pathological grades,different clinical stages and different histological types (P > 0.05).Conclusion The OPN and CXCR4 expression levels are correlated with the degrees of malignancy in epithelial ovarian tumor,therefore the CXCR4 and OPN expression pathways may be the new targets for ovarian cancer therapy.
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Objective To investigate the expression and clinical significance of platelet-derived growth factor A (PDGF-A) on placenta tissue from pre-eclampsia.Methods The expression of PDGF-A in the placenta of 38 pre-eclampsia patients and 22 normal pregnant women at third trimester was detected by immunohistochemistry method.Results (1)PDGF-A was mainly expressed in the cytomembrane and cytoplasm of cytotrophoblasts and the endothelial cell of capillary in placenta.(2) The rates of PDGF-A expression of cytotrophoblasts were 63% (24/38) in pre-eclampsia group and 32% (7/22) in normal pregnancy group,which exhibited significant difference (P ＜0.05).(3) The rates of PDGF-A expression of endothelial cell were 68% (26/38) in pre-eclampsia group and 27% (6/22) in normal pregnancy group,which also showed significant difference (P ＜0.01).(4)The rates of PDGF-A expression of cytotrophoblasts were 39% (7/18) in mild pre-eclampsia patients and 85% (17/20) in severe pre-eclampsia,which reached statistical difference (P ＜ 0.01).Conclusion The increasing expression of PDGF-A in cytotrophoblast and endothelial cell in placenta might confer the occurrence and progression of preeclampsia.
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Objective To detect the polymorphism of CYP1A1-MspI gene in patients with ovarian cancer.and discuss the relationship between the polymorphism ofCYP1A1-MspI gene and correspond cases' general materials and clinical materials.Methods The free peripheral blood samples of 81 cases confirmed to be ovarian cancer by postoperative pathology were collected preoperatively and the polymorphism of CYP1A1-MspI gene was detected.The clinical materials of the 81 cases with different genotypes were compared.The relationship between the polymorphism and clinical materials was analyzed.Results Among the 81 cases of ovarian cancer,there were 47 cases of wild type-genotype A(T/T)(58%),25 cases of mutation heterozygosis-genotype B(T/C)(31%),and 9 cases of mutation homozygosis-genotype C(C/C)(11%).The genotypic frequency distribution in patients aged from 12 to 29 was one case of genotype A(2.1%),5 cases of genotype B(20.0%),and no case of genotype C.The genotypic frequency distribution in patients aged from 30 to 49 was 12 case of genotype A(25.5%),8 cases of genotype B(32.0%),and 3 cases of genotype C(33.3%).The genotypic frequency distribution in patients aged from 50 to 69 was 31 case of genotype A(66.0%),8 cases of genotype B(32.0%) and 4 cases of genotype C(44.4%).The genotypic frequency distribution in patients aged more than 70 years was 3 case were of genotype A(6.4%),4 cases of genotype B(16.0%),2 case of genotype C(22.2%).There were significant differences of the ages of onset between patients with different CYP1A1-MspI genotypes (P
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Objective: To optimize the extraction process for Jiangmeiling Capsule(Fructus Schisandrae Chinensis). Methods : Uniformity design was used in extraction adoption with schisandrin, schisantherin, deoxyschisandrin, -schisandrin as marker. Results : The optimum process was that 95% alcohol used as extraction solvent, macerated twice for 20h and 10h, respectively, the amount of solvent added up to 10 and 9 times of herbs. The extraction efficiency was 97.47%, 95.81%, 93.77% and 95.82%, respectively. Conclusion : The extraction process was stable. The experimental results provided the basis for ascertainment of Jiangmeiling Capsule.
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AIM: To establish a HPLC method for the determination of schisandrin、 deoxy-schisandrin and ?-schisandrin in Jiangmeiling Capsule(extract of Fructus Schisandrae Chinensis). METHODS: The determination was performed by RP-HPLC on Kromasil TM C 18 column(200mm?4.6mm, 5?m) using methanol-H 2O (75∶25) as a mobile phase, flow rate at 1.0mL?min -1, detection wavelength at 224nm. RESULTS: The linear range of schisandrin was 0.02228～0.24508?g,r=0.9996. The average recovery was 101.87%, RSD=1.37% (n=5). The linear range of deoxyschisandrin was 0. 02188～0. 24068?g, r=0.9997. The average recovery was 99.75%, RSD=0.94% (n=5). The linear range of ?-schisandrin was 0.01975～0.2172?g, r=0.9996. The average recovery was 100.90%, RSD=0.99% (n=5). CONCLUSION: The method is convenient, sensitive and accurate. It can be a method for quality control in production of Jiangmeiling capsule.