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Objective To explore the effects of curcumin on the expression of C-erbB-2, Survivin and Bax in prost cancer cell. Methods The effects of curcumin on the expression of C-erbB-2, Suevivin and Bax were observed by SP immnohistocherminstry and cell cultivate methods. Results The expression of Survivin and C-erbB-2 in contrast group were strong, but the expression of Bax was very weak. After the curcumin were incubated with culture medium for 6h, 12h, 24h, 48h, the expression of C-erbB-2 and Survivin were gradually decreased in DU145 cells, while the expression of Bax was increased and reached highest at the time point of 12 ～24h. Conclusion The curcumin can reduce the expression of C-erbB-2 and surviving, and increase expression of Bax. The curcumin may exert antitumor activity by interacting with DU145 cells.
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BACKGROUND: Extract of astragalus mongholicus has good effects on antioxidization and oxygen free radical scavenge. Bone marrow mesenchymal stem cells (BMSCs) have multiple directional differentiation potential and superiority of autologous transplantation. This can provide a new way for treating neurodegenerative diseases.OBJECTIVE: To explore the differentiation of BMSCs of rats following astragalus mongholicus induction.DESIGN: A cytological in vitro study.MATERIALS: One clean male rats aged 6 weeks were purchased from Experimental Animal Center of Chinese Academy of Medical Sciences. Astragalus mongholicus injection (lot number 060105) were obtained from Dali Pharmaceutical Co., Ltd.METHODS: Rat BMSCs were in vitro isolated, cultured and purified by the whole bone marrow method. At the fourth passage, BMSCs at a density of 4×10<'8>/L were incubated in 12-well plate with a coverslip. After 80% 90% cells were confluent, the medium was changed. BMSCs were then incubated in DMEM, supplemented with 200 g/L astragalus mongholicus injection and 15% fetal bovine serum, for 6 days. BMSCs in the control group were not treated with astragalus mongholicus injection.MAIN OUTCOME MEASURES: Morphological changes in BMSCs were observed under the inverted microscope before and after induct.ion. Expression of specific markers was determined by immunocytochemical staining following induction.RESULTS: Primary cultured BMSCs were round. At the fourth passage, BMSCs were spindle. BMSCs after induction showed processes, which became more with prolonged time. Some processes were branch-shaped, forming a network structure. Results of immunocytochemical staining demonstrated that many cells were positive for nestin, neuron specific enolase and glial fibrillary acidic protein on day 3, and abundant cells were positive for microtubule-associated protein-2 on day 6.CONCLUSION: Astragalus mongholicus induces differentiation of BMSCs into neural stem cells, and then promotes the differentiation into neurons or glial cells, and makes differentiated cells mature and aging.
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BACKGROUND: Recently, Chinese herb and comprehensive therapy are widely adopted for the treatment of chronic atrophic gastritis (CAG), while infrared ray is widely used in the fields of physical therapy and scientific research. Therefore, some scholars suggest whether the physical characteristics of infrared ray have effects on the treatment of chronic atrophic gastritis.OBJECTIVE: To observe the effect of infrared ray on the changes of gastric mucosa tissue in rat models with chronic atrophic gastritis.DESIGN: Randomized controlled observation.SETTING: Hebei North University.MATERIALS: Thirty-five adult Wistar male rats weighing from 180 to 230 g were purchased from Hebei Experimental Animal Center [SCXK (ji) 2003-1-003]. The experiment was disposed with the ethical standard. Sodium salicylate powder produced by Beijing Fangcao Chemical Company (batch number: 890720). The drug was prepared with distilled water. Infrared lamp (220 V, 200 W) was bought by Equipment Division of our college.METHODS: The experiment was carried out in the Experimental Center of Hebei Beifang College from June 2005 to January 2006. ① Experimental intervention: Rats were fed with conventional standard granules for one week. Among them, 8 rats were selected as the normal control group, and other rats underwent model establishment. Rats were perfused with sodium salicylate and alcohol to stimulate gastric mucosa, and then chronic CAG models were established for 8 weeks based on exertion, irregular diet and other factors. Five rats were randomly selected for the check of histopathology before the end of model confirmedly making, and then the model rats were randomly divided into model group and infrared group with 11 in each group. Infrared lamp (220 V, 200 W, 0.76–1.5 μm in wavelength) was used to vertically radiate at the gastric projective area of rats in the infrared group, once a day, ten minutes once for twenty days. The rats in normal group and model group were regularly breed. ② Experimental evaluation: The body mass was weighed every week in 1, 4, 9 and 12 weeks after modeling. The infiltration of inflammatory cells and thickness of gastric mucosa were observed under optic microscope.MAIN OUTCOME MEASURES: ① Changes of body mass; ② pathohistological changes of gastric mucosa.RESULTS: All 30 rats were involved in the final analysis. ① Changes of body mass: From the end of the 4th week, increasing percentage of body mass in the model group and infrared group was decreased gradually as compared with that in the normal group, and there was significant difference (P < 0.05). ② Pathohistological changes of gastric mucosa: Gastric mucosa of rats in the model group was thinner, and atrophic glands, notable inflammatory infiltration and partial intestinal metaplasia were observed under optic microscope. The thickness of gastric mucosa in the infrared group was significantly thicker than that in model control group, and there was significant difference (P < 0.01); the inflammatory cells in the infrared group were less than those in the model group, and there was significant difference (P < 0.05). Morphologic structure and volume of the parietal cells were all recuperated or closed to normal.CONCLUSION: Infrared ray can decrease thickness of gastric mucosa and reduce inflammatory cells of rats with chronic atrophic gastritis, and it has greatly therapeutic achievements.
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BACKGROUND: The quality of life(QOL) of patients with lung cancer is ffected due to depression, reduced lung function, subjective reduced body force, fatigue, and poor stamina, etc., and the survival of the patientswould be affected by complications or advanced stage cachexia.OBJECTIVE: To investigate the relationship between three gene proteins including p53, p27 and bcl-2 and the pathological characters of non-small cell lung cancer (NSCLC).DESIGN: An experimental trial by employing pathological specimens as subjects.SETTING: Department of respiration of a university affiliated hospital and the Central laboratory of a university.PARTICIPANTS: Totally 76 specimens of NSCLC after surgical resection between June 1997 and December 2002, which were all primary lung cancer without any other therapy.METHODS: The expression of three gene proteins in 76 NSCLC specimens was detected by SP immunohistochemical analysis.MAIN OUTCOME MEASURES: Positive expression of p53, p27 and bcl-2.RESULTS: Among 76 specimens, 28 cases(37%, 28/76) with excessive expression of p53, 34 cases(45%, 34/76) with excessive expression of p27, 37 cases(49%, 37/76) with excessive expression of bcl-2, and 7 cases with excessive expression in all three proteins. The positive expression of p53 elevated with the reduced gradation in differentiation; bcl-2 and p27positive expressions reduced with the reduced gradation in differentiation and there was significant difference between high-differentiation group and low-differentiation group( P ＜ 0. 05) . However, there was no significant relationship between the positive expressions of three proteins and the histological classification, lymph node metastasis, and pathological aging of lung cancer( P ＞ 0.05).CONCLUSION: The excessive expression of p53, p27 and bcl-2 genes might be related with the occurrence and development of NSCLC.
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<p><b>BACKGROUND</b>To study the relationship between the expression of p53, p27, bcl-2 protein and the clinical pathological characteristics of non-small cell lung cancer (NSCLC).</p><p><b>METHODS</b>Expression of p53, p27 and bcl-2 protein was detected in 76 NSCLC samples by immunohistochemistry.</p><p><b>RESULTS</b>The positive rate of p53, p27 and bcl-2 protein was 36.84% (28/76), 44.74% (34/76) and 48.68% (37/76) respectively, and 7 cases were positive for p53, p27 and bcl-2 protein. Positive rate of p53, p27 and bcl-2 protein was not related to the pathological type, lymph node metastasis, and TNM stage. The positive rate of p53 in higher differentiation group was significantly lower than that in lower differentiation group (P < 0.05). The positive rate of p27 and bcl-2 in higher differentiation group was significantly higher than that in lower differentiation group (P < 0.05).</p><p><b>CONCLUSIONS</b>Overexpression of p53, p27 and bcl-2 genes may play an important role in the oncogenesis and development of NSCLC.</p>
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Pancreas of 53 aborted fetuses ranging from 5 to 41 weeks were fixed in Bouin's fluid and embedded in paraffin, and the sections were stained with Alcian bluephloxine and Grimelius silver technique. The A, B and D cell of islet of Langerhans were demonstrated with immunohistochemical PAP method also. 14 pancreas among them were fixed in 2% glutaraldehyde and embedded in Epon 812, and observed with TEM. Typical A, B and D islet cells were observed with LM at 12 weeks of fetal age. Undifferentiated, differentiated and degenerated cells were distinguished in the pancreatic islets at 14 to 41 weeks of fetal age. The secretory granules of the exoerine cells were observed after 14 weeks of fetal age. The functional differentiation of endocrine cells precede that of exocrine cells. The morphometrical measurement testified that the endocrine portion of fetal pancreas was obviously larger than that of adult. This study provides some morphological data for the pancreas transplantation in clinical medicine.