RÉSUMÉ
Aim: In this study, the effects of alpha-tocopherol (AT), quercetin (QT) or their combination on ethanol-induced pancreatic and duodenal mucosal damage were investigated in rats using morphological and biochemical evaluations.Study Design: Experimental study.Place and Duration of Study: University of Ibadan, Ibadan, Nigeria.Methodology: Ethanol-induced injuries were produced by oral administration of 40% ethanol (0.2 ml/day) for 40 consecutive days, while a control group of rats was served distilled water. Other groups received AT (2.5 mg/kg), QT (50 mg/kg) or their combination with 40% ethanol during the experimental period.Results: Blood glucose level was significantly (p<0.05) increased in ethanol-treated rats relative to controls. Ethanol administration caused shrinkage of insulin-secreting islets tissues in the pancreas, while lesions such as erosions, loss of villi and severe inflammatory cell infiltrations of the mucosa and sub-mucosa were observed in the duodenum. These changes were accompanied by significant elevation in the levels of hydrogen peroxide (H2O2), malondialdehyde (MDA) and advanced oxidation protein products (AOPP) in the pancreas and duodenum, along with reduced activities of glutathione peroxidase (GPx) and glutathione S-transferase (GST). Treatment of rats with AT, QT, and especially their combination, yielded profound reversal of ethanol-induced effects indicated by restoration of blood glucose to control levels, preservation of pancreatic and duodenal morphology and the inhibition of ethanol-induced oxidative stress.Conclusion: Overall, dietary supplementation with AT and/or QT could potentially counteract the adverse effects associated with chronic alcohol consumption.
RÉSUMÉ
Aim: The effect of the methanolic extract of Ocimum gratisimum (OG) leaves on the tissue damage induced by ischemia-reperfusion (IR) injury in the rat intestine was investigated. Study Design: Randomized controlled experiment. Place and Duration of Study: Experimental Animal Unit and Faculty of Veterinary Medicine, University of Ibadan, Nigeria from March to May, 2013. Methodology: 18 rats were divided randomly into 3 groups of 6 rats each. Group I served as control; Group II had IR injury by laparotomy with clamping of the Superior mesenteric artery (SMA) for 30 minutes followed by removal of the clamp for 45 minutes; Group III was pre-treated orally for 7days with methanolic extract of Ocimum gratissimum (MEOG) followed by IR injury. Sections of the duodenum and ileum were cut for histopathological examination. The remaining tissues were processed for the determination of biochemical markers of oxidative stress including Hydrogen peroxide (H2O2), Malondialdehyde (MDA) and Glutathione concentrations. Results: Mean values of MDA and H2O2 levels were significantly elevated (P=0.004 and P=0.03, respectively) in intestinal tissues following IR compared to control while reduced glutathione (GSH) levels were not significantly altered. OG (500mg/kg) caused significant reduction (P=0.02 and P=0.04) in MDA concentrations and H2O2 generation, respectively compared with the IR group. Histopathological examination revealed erosions and stunting of the villi tips in the duodenum and ileum, with severe mononuclear infiltration at the mucosal surface in the IR group. No visible lesions were observed in the intestine of the control group, with no significant alterations in the intestinal epithelium of the OGtreated rats. Conclusion: The results suggest that OG may provide some protection against intestinal mucosal injury induced by ischemia-reperfusion, through its anti-oxidative effects.