Identification of a gene set associated with colorectal cancer in microarray data using the entropy method

Objective: We sought to apply Shannon's entropy to determine colorectal cancer genes in a microarray dataset

Materials and Methods: In the retrospective study, 36 samples were analysed, 18 colorectal carcinoma and 18 paired normal tissue samples. After identification of the gene fold-changes, we used the entropy theory to identify an effective gene set. These genes were subsequently categorised into homogenous clusters

Results: We assessed 36 tissue samples. The entropy theory was used to select a set of 29 genes from 3128 genes that had fold-changes greater than one, which provided the most information on colorectal cancer. This study shows that all genes fall into a cluster, except for the R08183 gene

Conclusion: This study has identified several genes associated with colon cancer using the entropy method, which were not detected by custom methods. Therefore, we suggest that the entropy theory should be used to identify genes associated with cancers in a microarray dataset

[Outcomes of rectopexy of rectal prolapse through anus by sub mucosal injection of 5% dextrose]

Considering the vast methods of rectal prolapsed, a very common condition in children, either surgical [by abdominal or perineal approach] or non-surgical [sclerozing agents' injection], the authors evaluated the therapeutic effects of injecting dextrose 50% through perineal rectopexy. All those referred patients with rectal prolaps to Dr. sheikh children hospital were included in this study. All were sub-mucusally injected 5 cc of dextrose 50% solution and the therapeutic results [recurrence and complications] were evaluated. During this study 46 patients were injected about 5 cc of dextrose 50% solution. All went through the operation without any complications and during a 6 month following up none of the complications such as fistulae, abscess formation, urinary retention, and mucosal necrosis were detected. Nine patients were re-injected due to the prolapsed recurrence however no complications occurred in the second operation. The described procedure is a simple, inexpensive, and repeatable one. Deflux has been suggested as the best sclorizing agent in articles but it is expensive. Sub-mucusally injection of dextrose 50% is a simple, inexpensive, and repeatable procedure with a 100% positive therapeutic results, therefore could be, suggested as a substitution procedure in treating children's rectal prolapsed