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Braz. j. microbiol ; 44(2): 493-498, 2013. tab
Article Dans Anglais | LILACS | ID: lil-688584

Résumé

Staphylococcus aureus is one of the most frequent mastitis causative agents in small ruminants. The expression of most virulence genes of S. aureus is controlled by an accessory gene regulator (agr)locus. This study aimed to ascertain the prevalence of the different agr groups and to evaluate the occurrence of encoding genes for cytotoxin, adhesins and toxins with superantigen activity in S. aureus isolates from milk of ewes with clinical and subclinical mastitis in sheep flocks raised for meat production The agr groups I and II were identified in both cases of clinical and subclinical mastitis. Neither the arg groups III and IV nor negative agr were found. The presence of cflA gene was identified in 100% of the isolates. The frequency of hla and lukE-D genes was high -77.3 and 82.8%, respectively and all isolates from clinical mastitis presented these genes. The sec gene, either associated to tst gene or not, was identified only in isolates from subclinical mastitis. None of the following genes were identified: bbp, ebpS, cna, fnbB, icaA, icaD, bap, hlg, lukM-lukF-PV and se-a-b-d-e.


Sujets)
Animaux , Protéines bactériennes/génétique , État de porteur sain/médecine vétérinaire , Mastite/médecine vétérinaire , Maladies des ovins/microbiologie , Infections à staphylocoques/médecine vétérinaire , Staphylococcus aureus/génétique , Transactivateurs/génétique , Facteurs de virulence/génétique , Brésil , État de porteur sain/microbiologie , Gènes , Génotype , Mastite/microbiologie , Lait/microbiologie , Ovis , Infections à staphylocoques/microbiologie , Staphylococcus aureus/classification , Staphylococcus aureus/isolement et purification
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