Résumé
The phytochemical screening and antioxidant potential of bark and stem of Parthenocissus quinquefolia [L.]planch was assessed in order to verify its ethnopharmacological significance. All major secondary metabolites e.g. alkaloids, flavonoids, saponins, terpenoids, tannins, reducing sugars, cardiac glycosides and anthraquinones were present. Antioxidant activity was analysed by using five techniques which included DPPH Free Radical Scavenging Activity, FRAP [Ferric Reducing Antioxidant Power], TAA [Total Antioxidant Activity, TPC [Total Phenolic Content and MC [Metal Chelating] Activity. Ethanolic extract of bark showed the highest scavenging effects of 90.01+/-0.01%, with IC50 value of 24.32mg/ml. Aqueous stem extract showed best activity with IC50 value of 13.6+/-0.34mg/ml. The significance antioxidant potential indicates the effectiveness of bark and stem of P. quinquefolia in treatment of many diseases
Résumé
In the present study the crude extracts of pollen of Centella asiatica [Linn.] Urban were explored for their antioxidant potential using Ferric Reducing Power, Metal Chelating Activity and Trolox Equivalent Antioxidant Capacity assays. In crude extracts of pollen antioxidant components were initially extracted in methanol and further fractionated in solvents of different polarity, such as n-Hexane, Chloroform, Ethyl Acetate and Water exhibited reasonable antioxidant activity. The extract was found to contain large amounts of phenolic and flavonoid contents ranged from 143-1155 mg/1 of gallic acid equivalent [GAE] and 911-2488 mg/1 of quercetin [QE] respectively. Moreover, Super oxide Anion Radical Scavenging Activity and GS-MS analysis were also carried out