RÉSUMÉ
Correlated with such hepatic-systemic factors as cirrhosis, inflammation and immunity, portal vein thrombosis (PVT) is common in perioperative period of liver transplantation (LT) recipients.It affects negatively surgical procedures and outcomes due to its insidious onset and atypical clinical symptoms.With continuous improvements of LT techniques and refining of medical imaging, researchers have gained further insights into the pathophysiological processes, screening, diagnoses, evaluations, classifications and perioperative managements of PVT.This review focused upon perioperative managements of LT recipients with PVT to enhance the clinical problem-solving capability and long-term patient survival.
RÉSUMÉ
Objective:To explore the effects of novel coronaviral infection on the outcomes of liver transplantation recipients.Methods:The relevant clinical data were retrospectively reviewed for 5 liver transplantation recipients infected with novel coronavirus during perioperative period at First Hospital of Shanxi Medical University.At the same time, "liver transplantation" and "novel coronavirus" and "2019 novel coronavirus" or "SARS-CoV-2" were employed as Chinese and English keywords respectively for searching the relevant literature items of CNKI, Wanfang and PubMed from the inception of database until February 2023.The impact of perioperative novel coronaviral infection was examined on the outcomes of liver transplantation recipients.Results:From November 2022 to January 2023, liver transplantation was performed in 5 recipients of donated liver after death.Two cases tested positive for real-time polymerase chain reaction(RT-PCR)at Day 14/20 pre-operation and converted into negative post-operation.Three cases were infected with SARS-CoV-2 at Day 1/10/16 post-operation.And one of them became feverish again at Day 11 post-infection.With a peak temperature of 39.5 degrees, it rapidly progressed into multiple organ failures of heart, lung and kidney.After active interventions, organ function gradually recovered.Through literature searches, 9 eligible English literature items were retrieved.However, there was no Chinese entry.Among 15 liver transplantation recipients with SARS-CoV-2 infection, one died from septic shock, another from graft failure and the remainders survived.Conclusions:liver transplantation recipients infected with SARS-CoV-2 and negative RT-PCR pre-operation are asymptomatic.Without signs of pneumonia on chest CT, transplantation may be performed.For post-liver transplantation recipients with early SARS-CoV-2 infection, close monitoring of important organ functions such as heart, lung, liver and kidney, timely antiviral regimen and tapered use of immunosuppression can achieve a decent prognosis.
RÉSUMÉ
The open reading frame of Spinacia oleracea Betaine Aldehyde Dehydrogenase (SoBADH) was retrieved from Spinacia oleracea and inserted into the Agrobacterium tumefaciens binary vector pBin438, which was driven by CaMV35S promoter, and produced the new binary vector pBSB. A. tumefaciens LBA4404 carrying this plasmid was used in genetic transformation of plants. Forty-five primary transgenic plants were detected by PCR and verified by the Southern blotting from 65 regenerated plants, of which 27 transgenic plants had only one copy of T-DNA. The Northern blotting and Western blotting analysis indicated that the SoBADH gene had been transcribed mRNA and expression protein in the transgenic cotton lines. The testing of SoBADH activity of transgenic plant leaves showed that the enzyme activity was much higher than that of the non-transgenic cotton. The growth of transgenic plants was well under the salinity and freezing stress, whereas the non-transgenic plant grew poorly and even died. Challenging with salinity, the height and fresh weight of transgenic plants was higher compared with those of non-transgenic plants. Under the freezing stress, the relative conductivity of leaf electrolyte leakage of the transgenic cotton lines was lower than that of non-transgenic plants. These results demonstrated that the SoBADH gene could over express in the exogenous plants, and could be used in genetic engineering for cotton stress resistance.