Frequency of the hypervariable DNA loci D18S849, D3S1744, D12S1090 and D1S80 in a mixed ancestry population of Chilean blood donors

Blood donors (N = 150) at San José Hospital (Santiago, Chile) were typed for one VNTR locus (D1S80) and three STR loci (D18S849, D3S1744, D12S1090). A questionnaire was used to determine the socioeconomic level of the donors, because it is known that some genetic markers (e.g., the ABO and Rh groups) are differentially distributed between different socioeconomic strata. This methodology revealed that two of the three socioeconomic strata distinguishable in Santiago were present in our sample of blood donors, with stratum II representing the middle strata and stratum III the low strata. Allele frequency was determined for each locus and socioeconomic stratum, and it was found that the allele distributions of each locus in socioeconomic strata II and III were statistically similar. All loci conformed to the Hardy-Weinberg law and there was no evidence for association between the alleles of the four loci, allelic frequencies being similar to those found in North American Hispanic populations. The results support the view that the analysis of these loci may have useful applications in population genetics as well as in identity tests

Estudio comparativo de sueros antiglobulina humana de uso en Chile. / Comparative study of human antiglobulin serum used in Chile

Se hace un estudio comparativo de cinco sueros antiglobulina humana de amplio espectro o poliespecifico de uso mas frecuente en el pais, procedente de diferentes laboratorios. Se valora su contenido de anti IgG por medio de hematies D positivo y Duffy a (Fya) positivo, sensibilisados con Anti D Anti Fya, respectivamente y su actividad anticomplemento referida principalmente a su reactividad Anti C3d necesaria para el test directo de antiglobulina, cuando se estudian anemias hemoliticas autoinmune