Lipid peroxidation and DNA damage in different stages of acute renal failure.

Introduction: Oxidative stress is an imbalance between the formation of Reactive oxygen species and protective antioxidant defense. It is known that oxidative stress increases the Acute renal failure. Measurement of oxidative stress parameters may be a simple tool for monitoring the progression of acute renal failure. Aim: The aim of this study was to evaluate oxidative stress in acute renal failure patients by Lipid peroxidation product Malondialdehyde (MDA), Total antioxidant capacity (TAC) and oxidative DNA damage product and compare its level among the patients of varying severity as per RIFLE classification. Materials and methods. We conducted a cross sectional study to compare oxidative stress parameters. Blood samples were collected from 62 patients with ARF, admitted to the Vinayaka Mission’s Medical College & Hospital, Salem from March 2009 to July 2010. We further subdivided the patients according to RIFLE classification. Results: The levels of MDA, Index of oxidative status MDA/TAC and DNA damage product 8 OH deoxy guanosine were significantly higher among failure group when compared to risk and injury. Total antioxidant capacity and super oxide dismutase (SOD) were found to be decreased.

Oxidative stress, interleukin (IL-6) and atherogenic index of plasma in diabetic nephropathy.

Plasma IL-6 and serum MDA were significantly high in microalbuminuria and macroalbuminuria as compared to normal controls and normoalbuminuria. Plasma TAC was significantly decreased in all the groups of diabetic patients as compared to normal controls. Significant increase in lipid parameters and AIP were observed in macroalbuminuria and microalbuminuria as compared to normal controls. Plasma IL-6 showed negative correlation with HDL-c (r=-0.255) and significant positive correlation was observed between MDA and HbA1c (r=0.537) in diabetic patients. Conclusion: Increased IL-6, MDA levels and decreased plasma TAC levels in diabetic patients revealed inflammation with increased oxidative stress, which may involve in the development of renal damage. The associated altered lipid profile and high risk AIP indicates the risk of developing cardiovascular complications in diabetic patients with macroalbuminuria.