Résumé
Eleven fungal strains were tested for their ability to produce brown and reddish brown textile dyes using H-acid (1- naphthol-8-amino-3, 6-disulfonic acid) as a dye precursor in the fermentation medium. All tested fungal strains exhibited high ability to produce dyes varying in both dye color (brown to reddish brown) and fastness properties to washing, perspiration and UV light. The produced dyes were subjected to further analysis for quantitative determination of dye components for investigation of their inter-relations as well as their role in dye color and stability.
Résumé
In a screening of fungal isolates associated with marine algae collected from Abou-keer, Alexanderia during the four seasons of 2004, to obtain new biologically active compounds. Varicosporina ramulosa isolate was identified and selected as a producer of 13 compounds. Out of 13 pure compounds produced, compounds 3 and 10 were considered as antibacterial and antifungal compounds, respectively as they were active against gram positive, gram negative bacteria and a fungus. Optimization of conditions (fermentation media, incubation period, temperature, initial pH, aeration levels) which activate compounds 3 and 10 production were studied. Also the spectral properties (UV, MS, GC/MS, IR and 1H-NMR) of the purified compounds were determined. Compound 3 suggested to be dibutyl phthalate and compound 10 may be ergosterol or one of its isomers. Biological evaluation of the two compounds towards 6 different types of tumor cell lines showed weak effect of compound 3 at different concentrations on the viable cell count of the different tumor cell lines. While compound 10 showed different activities against the viable cell count of the 6 different tumor cell lines. It kills 50% of the viable infected liver and lung cells at concentrations equal to 99.7 μg/mL, 74.9μg/mL, respectively. Compound 10 can be recommended as new anticancer compounds.