Résumé
Breast cancer is a major health problem that affects more than 24% of women in Bangladesh. Further- more, among low-income countries including Bangladesh, individuals have a high risk for developing breast cancer. This study aimed to identify candidate mitochondrial DNA (mtDNA) biomarkers for breast cancer diagnosis in Bangladeshi women to be used as a preventive approach. We screened the blood samples from 24 breast cancer patients and 20 healthy controls to detect polymorphisms in the D-loop and the ND3- and ND4-coding regions of mtDNA by direct sequencing. Among 14 distinct mutations, 10 polymorphisms were found in the D-loop, 3 were found in the ND3-coding region, and 1 was found in the ND4-coding region. The frequency of two novel polymorphisms in the D-loop, one at position 16290 (T-ins) and the other at position 16293 (A-del), was higher in breast cancer patients than in control subjects (position 16290: odds ratio = 6.011, 95% confidence interval = 1.2482 to 28.8411, P = 0.002; position 16293: odds ratio = 5.6028, 95% confidence interval = 1.4357 to 21.8925, P = 0.010). We also observed one novel mutation in the ND3-coding region at position 10316 (A > G) in 69% of breast cancer patients but not in control subjects. The study suggests that two novel polymorphisms in the D-loop may be candidate biomarkers for breast cancer diagnosis in Bangladeshi women.
Sujets)
Adulte , Sujet âgé , Femelle , Humains , Adulte d'âge moyen , Bangladesh , Marqueurs biologiques tumoraux , Métabolisme , Tumeurs du sein , Diagnostic , Génétique , Amorces ADN , Génétique , ADN mitochondrial , Génétique , Mutation , Odds ratio , Réaction de polymérisation en chaîne , Polymorphisme génétiqueRésumé
Chittagong, Cox's Bazar and Chittagong Hill districts of Bangladesh were visited to know the traditional uses of orchids both epiphytic and terrestrial, used by the tribal people of these regions. Indigenous knowledge on different orchid species has been documented during the ethnobotanical survey by taking random interviews of herbalists and elderly people among the tribal communities viz. Chakmas, Tripuras, Maramas and Tanchinga. The family Orchidaceae is represented in Bangladesh by 160 species with 2 varieties. Of them, 26 orchid species were found ethnobotanically important and most of them were used for medicinal purposes. Some of them were used for fishing, dyeing, packaging and other purposes. Voucher specimens for each studied orchid species were collected, identified and preserved in the Herbarium of Chittagong University