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Braz. j. microbiol ; 41(1): 59-65, Jan.-Mar. 2010. ilus, tab
Article Dans Anglais | LILACS | ID: lil-531735

Résumé

Food handlers, an important factor in food quality, may contain bacteria that are able to cause foodborne disease. The present study aimed to research coagulase-negative (CNS) and -positive staphylococci (CPS) in 82 food handlers, analyzing nasal and hand swabs, with identification of 62 CNS (75.6 percent) and 20 CPS strains (24.4 percent). Staphylococcal enterotoxins genes were investigated by PCR. In 20 CPS strains, 19 were positive for one or more genes. The percentage of CNS presenting genes for enterotoxins was high (46.8 percent). Despite of the staphylococcal species, the most common gene was sea (35.4 percent), followed by seh and sej (29.2 percent). The detection of new staphylococcal enterotoxins (SEs) genes showed a higher pathogenic potential in this genus. The presence of these gene points out the importance of CNS not only as contaminant bacteria but also as a pathogen.


Sujets)
Coagulase/analyse , Coagulase/isolement et purification , Entérotoxines/génétique , Entérotoxines/isolement et purification , Manipulation des aliments , Fosse nasale , Réaction de polymérisation en chaîne , Échantillons Alimentaires , Méthodes , Méthodes
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