Isolation and screening of bacteria with biofilm formation ability and characterization with hydrolytic enzyme production for enhanced biogas production 96-101

@#Aims: Biofilm is a complex structure that provides protection towards the bacteria within the barrier. Enhanced biogas production from Palm Oil Mill Effluent (POME) can be achieved by applying biofilm based anaerobic digestion system.Methodology and results:Bacteria that produces biofilm were isolated and tested on its hydrolytic enzyme secretion. The biofilm produced were also characterized. Out of 120 strains isolated from POME, PKC and food waste compost, only 33 strains were producing biofilm and only 11 of them exhibited significant amount of biofilm produced at optical density of wavelength 595 nm (>0.01). In hydrolysis enzyme assay test, all strains were not able to secrete protease enzyme. The biofilms were extracted and characterized to show similar characteristic for all strains. Strain numbers of 11, 9C, 23C and 30C showed positive result for cellulase, amylase and lipase enzymes, to be tested as single strain bacteria and also mixed with other isolated bacterium for prospect research on effective hydrolysis towards enhanced biogas production. The composition of biofilms from different bacteria mixture also similar under the same incubation condition.Conclusion, significance and impact of study: Bacteria producing biofilm are very limited and does not secrete the same hydrolytic enzymes. Utilization of these bacteria may eliminate the problem of microbial instability in a system.

Wastewater treatment by microalgae-bacteria co-culture system

Aims@#Microalgae is one of the bioremediation agents in wastewater treatment due to its ability to degrade nutrients and organic compounds. Several studies reported that co-cultivation of microalgae and bacteria, i.e. Microalgae Growth Promoting Bacteria (MGPB) could improve the nutrients removal process. This MGPB helps to degrade complex nutrient compounds into smaller components before being taken up by microalgae. The objective of this study is to investigate the effectiveness of co-cultured (microalgae and bacteria) system compared to axenic microalgae system in the removal of major nutrients (ammonium and phosphorus) and chemical oxygen demand (COD) in synthetic wastewater. @*Methodology and results@#In this study, two different strains of microalgae (Chlorella vulgaris and Scenedesmus quadricauda) were each co-cultured with a MGPB (Azospirillum brasilense) and their effectiveness in the removal of major nutrients and COD were compared. The results show that, the nutrients and COD removal were higher in co-cultured system compared to the axenic microalgae under similar cultivation conditions for both microalgae strains. Higher percentage removal was obtained from co-cultured C. vulgaris compared to that from co-cultured S. quadricauda which were 86% and 48%, 44% and 31%, 62% and 35% for ammonium, phosphorus and COD removal respectively. @*Conclusion, significance and impact of study@#The findings of this study demonstrate the potential of the co-culture of C. vulgaris and A. brasilense to be applied in wastewater treatment, specifically replacing the aerobic digestion process in secondary stage of conventional wastewater treatment. This study provides an important insight into developing an efficient and environmental friendly method to treat wastewater by incorporating the green technology in the treatment system.