Protection against methanol intoxication in rat liver enzymes by vitamins C and E

Antioxidant [AO] effects of vitamins C and E on the alteration of some enzymes of glucose metabolism induced by repeated doses of methanol were studied in rat livers. The selected enzymes include hexokinase [HK], glucose phosphate isomerase [GPI] and pyruvate kinase [PK] as glycolytic enzymes as well as fructose 1,6-diphosphatase [F-1,6 DP] as gluconeogenic enzyme. The results showed that methanol caused a pronounced decay in the activities of the all measured enzymes. AO vitamins administration showed a high protective effects indicated by significant increase in the activities of these enzymes. This may be attributed to the inhibition of free radical production induced by toxic effect of methanol with subsequent minimum degree of tissue damage

Susceptibility of biomphalaria Alexandria to infection with schistosoma mansoni: Correlation with the activity of certain glycolytic enzymes

The importance of the glycolytic flux for the success of Biomphalaria- Schistosome sporocyst interaction was acertained in this study. Hexokinase [HK], pyruvate kinase [PK], glucose phosphate isomerase [GPI] and lactate dehydrogenase [LD], as four important glycolytic enzymes, were markedly stimulated in trematode infected Biomphalaria alexandrina when measured two weeks post exposure to infection with Schistosoma mansoni miracidia. Treatment with this plant resulted in a significant inhibition of these three investigated enzymes. LC10 concentrations of S. nigrum reduced considerably the infection rate of B. Alexandrina with S. mansoni to be 34% compared to an infection rate of 80% in control, non-treated snails. Susceptibility of B. alexandrina to infection with the digenetic trematode S. Mansoni is correlated to the activity levels of the glycolytic enzymes. Moreover, sublethal and less pollutant concentration of S. Nigrum could be recommended to control schistosomiasis by disturbing the intramolluscan environment of the parasite