[Antibacterial activity of total extract, petroleum ether, chloroform, ethyl acetate and aqueous fractions of aerial parts of heliotropium bacciferum]

Heliotropium bacciferum is one of the plants belonging to the family Boraginaceae, which is Restricted distribution in the south of Iran. It is used for Hypotension, fever, stomach ulcers in traditional medicine. In this study, the antibacterial effects of extracts and fractions of chloroform, ethyl acetate and aqueous, aerial parts of Heliotropium bacciferum Forssk was evaluated against five bacterial strains. The methanol extract were prepared using the percolation method. Fractions of chloroform, Petroleum ether, ethyl acetate, methanol and aqueous respectively by Liquid - Liquid fractionation of the total extract were prepared. The antibacterial activity against two Gram positive bacteria, three Gram negative bacterial using Minimum inhibitory concentration in microplate and well plate method. Results showed that H. bacciferum extracts exhibited a significant activity against strains Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa, E.coli and Salmonella enteritidis. MIC and well plate is between 7.6-125 micro g/ml. The results of this study indicate that extracts of the plant H.bacciferum has a antimicrobial effect against strains are listed And among the extracts, aqueous part is that most antibacterial effect of the other fraction and then methanolic extract has the greatest effect

Effect of a static magnetic field on bone healing in the dog: radiographic and histopathological studies

Although the promotional effects on bone healing of pulsed electromagnetic fields [PEMF] have been well demonstrated, the effects of static magnetic fields [SMF] remained unclear. In this study, effects of a custom-made magnetic wrap on radiographic and histopathological aspects of bone healing using a canine unstable osteotomy gap model were investigated. After an osteotomy of the midshaft radius, bone healing was evaluated over an 8-week-period in the control dogs [n = 5] and dogs exposed to SMF [1000 gauss] [n = 5]. Bone healing was evaluated by qualitative and quantitative evaluation of serial radiographs every 2 weeks. Histopathological study was also performed on osteotomized radii upon completion of the experimental period. Dogs exposed to SMF had more advanced radiographic healing of osteotomy sites. Cellular morphology scores of the SMF group was significantly greater as compared with the control group [P<0.05]. These results suggested that using the osteotomy gap model, SMF enhanced the radiographic and histopathological aspects of bone healing in dogs. Dogs at risk for delayed healing of fractures may benefit from treatment with SMF