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Chinese Journal of Oncology ; (12): 111-114, 2003.
Article Dans Chinois | WPRIM | ID: wpr-347482

Résumé

<p><b>OBJECTIVE</b>To screen out the HAb18G/CD147 binding peptides and find out an antagonist against hepatoma invasion.</p><p><b>METHODS</b>HAb18G/CD147 was purified by affinity chromatographic method and the antigen binding peptides acquired by bio-panning a phage-displayed 12-peptide library. After obtaining the sequence of the selected phage-displayed peptides, all the 9 peptides were synthesized by solid-phase method and identified by mass spectrograph. The peptides' anti-metastatic function was tested by Boyden Chamber assay.</p><p><b>RESULTS</b>The purified HAb18G/CD147, identified by Western blot (molecular weight about 65 kd) could be used to bio-pan the phage-displayed peptide library. After 3 rounds of bio-panning, 9 positive phage clones were selected and sequenced. The synthesized peptides had uneven inhibitory activities and three of them were able to markedly inhibit the hepatoma cell invasion (P < 0.01). The most effective peptide decreased by 90.1% of hepatoma cells migrating through the Boyden Chamber membrane as compared with the control.</p><p><b>CONCLUSION</b>Bio-panning the phage-displayed peptide library can be used successfully to screen out the antigen binding peptides. Hepatoma metastatic potential can be inhibited by peptide antagonist which could be a good foundation of developing peptide therapeutic agent against hepatoma metastasis.</p>


Sujets)
Animaux , Humains , Souris , Anticorps monoclonaux , Utilisations thérapeutiques , Antigènes CD147 , Métabolisme , Carcinome hépatocellulaire , Traitement médicamenteux , Anatomopathologie , Tumeurs du foie , Traitement médicamenteux , Anatomopathologie , Invasion tumorale , Banque de peptides , Peptides , Utilisations thérapeutiques
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