Technical details of ultrasound guided mammotome minimally invasive biopsy system for benign breast mass in 186 cases / 中华普通外科杂志

Objective To explore the clinical results and operation experiences for benign breast mass by ultrasound guided mammotome minimally invasive biopsy system (MMIBS). Methods 212 benign breast masses in 186 patients were resected by ultrasound guided MMIBS. Clinical data of 186 patients were retrospectively analyzed. Results Needle position in 186 patients was visualized. Lesions were completely removed in 134 cases of 186 (72%) patients. The complete resection rate for tumors on major pectoral muscle or near areola were 31.5% (6/19) and 33.3% (4/12) respectively. Identified by postoperative ultrasound, 118 out of 134 patients (88.0%) with tumor sizes 0.5 to 2.5 cm and 16 out of 38 patients (42.1% ) with sizes 2.5 to 3.0 cm were completely removed. No lesions larger than 3.0 cm were completely removed. All 52 cases in which the tumors were not completely removed by MMIBS were converted to open surgery. Ultrasound follow-up after 4 weeks showed that all the 134 cases that had had masses completely removed had no residual masses, whereas 6 months after operation, 16 out of the 112 cases proved tumor recurrent necessitating open reoperation in 6 cases and second MMIBS operation in 10 cases, among them one case recurred after six months and received open operation. Conclusions For small benign breast mass, MMIBS has therapeutic effect with significantly minimal invasion.

Transplantation of corneal stem cells cultured on amniotic membrane for corneal burn: experimental and clinical study / 中华医学杂志(英文版)

<p><b>OBJECTIVE</b>To investigate the proliferation and differentiation of cultured corneal stem cells and determine the effect of corneal stem cells cultured on amniotic membranes on the limbal area for treating corneal burns.</p><p><b>METHODS</b>The proliferation and differentiation of corneal stem cells in vitro had been examined using colony-forming efficiency and immunohistochemistry. The stem cells had been cultured on amniotic membranes and transplanted to the limbal area for treating corneal burns.</p><p><b>RESULTS</b>Corneal stem cells had a high proliferation capacity in primary and first passage, cytokeratin 3 was not expressed in primary culture but partly in first passage. The stem cells could proliferate to form cell layer on an amniotic membrane. When transplanted, stem cells could survive on limbus. After transplantation, ocular inflammation resolved, the cornea re-epithelialized, the stromal opacity reduced, the superficial neovascularity was lessened and the conjunctival fornix re-established.</p><p><b>CONCLUSIONS</b>Ocular surface conditions could be improved by allograft of corneal stem cells cultured on amniotic membranes.</p>

Expression of mannose-receptor in cultured bovine iris pigment epithelial cells in vitro / 中华眼底病杂志

Objective To verify whether iris pigment epithelial cells (IPECs) possess the similar potential of specific phagocytosis to retinal outer segments (ROS) with retinal pigment epithelial cells (RPECs). Methods IPECs were isolated from neonatal bovines with Hu′s method, and were cultured. The cultured cells were identified by immunohistochemical methods with antibodies to cytokeratin and S 100. Total RNA of IPECs was extracted by Trizol. The specific primers for mannose receptor and ? actin were designed according to their sequence from Genbank. The mRNA expression of these proteins in the IPECs was analyzed by reverse transcription polymerase chain reaction (RT PCR). Results The cultured IPECs have no contamination of other cells. The extracted RNA was ideal and had no degradation. RT PCR analysis showed that mannose receptor′s mRNA was expressed in cultured IPECs in vitro. Conclusions Cultured IPECs may express the mannose receptor, and may have similar potential of phagocytosis to ROS with RPECs.