An Analytical Study Of Histopathological Findings Of 100 Cases Of Fistula-in-ano

Fistula-in-ano is one of the most difficult diseases confronted in ano-rectal patients in Shalyatantra OPD. Sushruta described Bhagandara along with its etiology, clinical presentation and treatment. The description of different types of Bhagandara (fistula-in-ano) are at par with the aetiopathogenesis of Fistula-in-ano of modern medical science. It is a very complicated condition arising out of various causes like pyogenic, tubercular, lymphogranuloma inguinale, inflammatory bowel diseases like Crohn's disease, ulcerative colitis etc. Identification of the aetiopathological factor is necessary for the success of treatment of fistula-in-ano. Histopathological study of tissue from tract of fistula-in-ano is an useful diagnostic tool for assessing the underlying pathology. In this study we use the benefits of histopathological examination from the primary site of the disease to detect the basic etiology. 100 cases diagnosed as fistula-in-ano attending Shalya OPD, Government Ayurvedic College Hospital were randomly selected for a period of one year i.e 2016-2017. After necessary investigations, tissue is collected from the primary site of fistulous tract during primary threading for Kshar Sutra therapy, in OT under local anaesthesia and sent for histopathological examination. The results of the above study were tabulated and lots of cases other than pyogenic cause were detected. The data were analysed with standard statistical methods. Without histopathological examination it is not possible to detect the underlying cause of fistula-in-ano.

Early onset primary pulmonary cryptococcosis in a renal transplant patient.

We report a case of primary pulmonary cryptococcosis in a post-renal transplant patient. A 65-year-old male renal transplant patient was admitted to the hospital with a low grade fever of 1 month, radiologically mimicking tuberculosis (TB). Broncho-alveolar fluid (BAL) shows capsulated yeast, and Cryptococcus neoformans was grown on culture supported by cytology and histopathological examination. Cryptococcal antigen was positive (32-fold) in serum and was negative in cerebrospinal fluid (CSF). The patient was given amphotericin B and 5-flucytosine and clinical improvement was seen on a weekly follow up. The serum cryptococcal antigen test might contribute to the early detection and treatment of pulmonary cryptococcosis. The results of antifungal susceptibility were aid in selecting the drug of choice for treatment.

A rare case of diphyllobothriasis from Pondicherry, South India.

Diphyllobothriasis is an intestinal parasitic infection caused by the ingestion of raw fresh-water fish containing the infectious larvae of Diphyllobothrium spp. This infection is uncommon in India. We report a case of diphyllobothriasis that occurred in Pondicherry, India, in a 5-year-old boy hailing from a fishing community. He attended the Pediatric OPD with spontaneous discharge of segments of the adult parasite. The segments (macroscopically and microscopically) were identified as those of Diphyllobothrium latum. The stool examination also revealed characteristic oval eggs.

An automated annotation tool for genomic DNA sequences using GeneScan and BLAST.

Genomic sequence data are often available well before the annotated sequence is published. We present a method for analysis of genomic DNA to identify coding sequences using the GeneScan algorithm and characterize these resultant sequences by BLAST. The routines are used to develop a system for automated annotation of genome DNA sequences.