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Mem. Inst. Oswaldo Cruz ; 103(2): 195-200, Mar. 2008. graf, tab
Article Dans Anglais | LILACS | ID: lil-480634

Résumé

Adenoviruses (AdV) are commonly involved in acute respiratory infections (ARI), which cause high morbidity and mortality in children. AdV are grouped in six species (A-F), which are associated with a wide range of diseases. The aim of this study was to identify the AdV species infecting non-hospitalized Mexican children with ARI symptoms, attending to the same school. For that, a PCR/RFLP assay was designed for a region of the hexon gene, which was chosen, based on the bioinformatical analysis of AdV genomes obtained from GenBank. A total of 100 children's nasopharyngeal samples were collected from January to June, 2005, and used for viral isolation in A549 cells and PCR/RFLP analysis. Only 15 samples produced cytopathic effect, and in all of them AdV C was identified. AdV C was also identified in eight additional nasopharyngeal samples which were negative for viral isolation. In summary, this outpatient population showed a rate of AdV infection of 23 percent, and only AdV C was detected.


Sujets)
Enfant , Femelle , Humains , Mâle , Infections humaines à adénovirus/virologie , Adénovirus humains/génétique , Infections de l'appareil respiratoire/virologie , Maladie aigüe , Infections humaines à adénovirus/diagnostic , Infections humaines à adénovirus/épidémiologie , Adénovirus humains/isolement et purification , Techniques de typage bactérien , DNA restriction enzymes/analyse , Marqueurs génétiques , Génome viral , Mexique/épidémiologie , Partie nasale du pharynx/virologie , Réaction de polymérisation en chaîne , Polymorphisme de restriction , Infections de l'appareil respiratoire/diagnostic , Infections de l'appareil respiratoire/épidémiologie , Saisons
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