Résumé
In the current study, we evaluated the mechanism of action of miltefosine, which is the first effective and safe oral treatment for visceral leishmaniasis, in Leishmania amazonensis promastigotes. Miltefosine induced a process of programmed cell death, which was determined by the externalization of phosphatidylserine, the incorporation of propidium iodide, cell-cycle arrest at the sub-G0/G1 phase and DNA fragmentation into oligonucleosome-sized fragments. Despite the intrinsic variation that is detected in Leishmania spp, our results indicate that miltefosine causes apoptosis-like death in L. amazonensis promastigote cells using a similar process that is observed in Leishmania donovani.
Sujets)
Antiprotozoaires , Apoptose , Fragmentation de l'ADN , ADN des protozoaires , Leishmania mexicana , Phosphoryl-choline/analogues et dérivés , ADN des protozoaires , Cytométrie en flux , Phosphoryl-cholineRésumé
El trabajo clínico comunitario posibilita descubrir, elaborar el imaginario social hecho texto grupal. Cuando digo imaginario social, me refiero a la definición que da Cornelius Castoriadis: conjunto de significaciones por las cuales un colectivo, una sociedad, un grupo, se instituye como tal inventando sus formas de relación, sus modos de contrato y sus figuraciones subjetivas.
Community clinical work makes it possible to discover, elaborate the social imaginary made group text. When I say social imaginary, I refer to the definition that Cornelius Castoriadis gives: a set of meanings by which a collective, a society, a group, is instituted as such by inventing its forms of relationship, its modes of contract and its subjective figurations.
Sujets)
Psychothérapie de groupe , Psychodrame , Psychologie sociale , Relations interpersonnellesRésumé
Partimos de la clínica de la vincularidad articulándola en cuatro ideas centrales: ética, poder, violencia y trasgresión. El grupo que instituimos para este trabajo, atravesó avatares y climas diversos en los que, estos cuatro conceptos eje, no solo fueron un tema de discusión, sino que estaban entramados en nuestras relaciones grupales.
We start from the clinic of the linkage articulating it in four central ideas: ethics, power, violence and transgression. The group that we instituted for this work, went through various avatars and climates in which, these four core concepts, were not only a topic of discussion, but were embedded in our group relationships.