Single-step bulk purification of synthetic tetrapeptide tuftsin by high performance liquid chromatography.

A protocol on reversed phase high performance liquid chromatogrpahy (RP-HPLC) using water-acetonitrile gradients under lesser stringent conditions, has been devised to obtain highly purified tuftsin in bulk amount. The protocol was also tested for two different preparations of tuftsin to yield identical quality of peptide.

Identification and size variation of terminal fragments of sheep pox virus genome.

Terminal fragments of sheep pox virus DNA identified by snap-back analysis showed terminal covalent cross-links. Southern blot hybridization using a terminal fragment probe confirmed the termini and terminal repeats (common sequences) of the sheep pox virus genome. Terminal fragment length variability was observed between virus isolates.