Résumé
Antigenic identity between the cell surface carbohydrate ligands and their excreted factor (EF) in all forms of Indian leishmaniasis were assessed by 11 carbohydrate-specific lectins. Our results demonstrated that 3 lectins, viz. PNA, SBA and WFA showed unique selectivity towards skin-dwelling parasites. The EFs of visceral leishmaniasis (VL), post-kala-azar dermal leishmaniasis (PKDL) and cutaneous leishmaniasis (CL) formed band against ConA at 1000, and 500 micrograms/ml concentration, respectively. RCA-120 at 3 mg/ml concentration was positive to both Leishmania tropica and L. major, whereas SBA was specific to L. tropica, L. major and PKDL strains at the same concentration. Antisera directed against the EFs (EF-As) induced agglutination to homologous promastigotes suspension. Cross-reactivity of agglutination was observed in different strains but highest was found among PKDL and CL strains. Although PKDL cases are normally found in patients with the history of visceral leishmaniasis (kala-azar), surprisingly in this study, the surface saccharides of PKDL strains had close affinity to CL type instead of VL.