Analysis of prescribing of antiplatelet drug in patients with coronary heart disease in department of geriatrics / 中国临床药理学与治疗学

AlM: To analyze the prescribing of different antiplatelet drugs and clinical conditions of patients with coronary heart disease in department of Geriatrics. METHODS: This study was a single center and cross-sectional study. Patients with coronary heart disease were recruited when hospitalized in the department of Geriatrics department, Xiangya Hospital, Central South University between December 2021 and June 2022. We investigated and analyzed the clinical conditions of patients including the prescribing of the antiplatelet drugs. RESULTS: A total of 347 coronary heart disease patients with a mean age of (65.2 ± 10.1) years were included. The antiplatelet drug clopidogrel was the most commonly prescribed. Among the different dual antiplatelet therapy (DAPT), aspirin combined with clopidogrel was the most widely used. With the increasing of the numbers of target coronary lesions, the prescribing rate of clopidogrel increased significantly (P < 0.05). In three groups with different degrees of target coronary lesions, the prescribing rate of aspirin was higher than that of indobufene (P < 0.05). Compared with patients taking aspirin, the patients taking indobufene were older with a higher blood creatinine level, a higher proportion of chronic gastropathy and the higher level of platelet aggregation rates (P < 0.05). CONCLUSlON: Clopidogrel is the most commonly prescribed antiplatelet drugs in patients with coronary heart disease. Aspirin combined with clopidogrel is the most widely used in DAPT. Compared with aspirin, indobufene is more commonly used in elderly patients with the chronic gastritis and a higher serum creatinine level.

Review of bleeding events in clinical studies of clopidogrel / 中国临床药理学与治疗学

P2Y12 receptor antagonist is currently one of the commonly used drugs for the treatment of acute coronary syndromes. Clopidogrel is a representative drug among P2Y12 receptor antagonists. Bleeding events as the most common side effect of this drug have also been concerned. The incidence and risk of bleeding events in large-scale clinical studies of clopidogrel are analyzed and discussed to provide refer-ences for reasonable clinical prescription of clopidogrel.

Exploration on online teaching of Geriatrics during the COVID-19 pandemic / 中华医学教育探索杂志

The opening of universities and colleges has been postponed due to the outbreak of COVID-19. In order to fully guarantee the normal teaching order and achieve the goal of "classes suspended but teaching and learning continues", Central South University has adopted online teaching in the spring semester of 2020. The Geriatrics teaching team of Xiangya Hospital have actively responded to the school's call and carried out online teaching course. This paper summarizes the experience in the preliminary organization, process implementation, education management and teaching evaluation of the Geriatrics online course in order to provide reference for the teaching reform of this course.

Quality Standard of Xiao'er Resuqing Granules / 医药导报

Objective To establish the quality standard of Xiao'er resuqing gantules.Methods Rhei Radix et Rhizoma,Bupleuri Radix,Forsythiae Fructus,Puerariae Lobatae Radix in the granules were qualitatively identified by the method of thin layer chromatography (TLC).The content of baicalin was analyzed by the method of high performance liquid chromatography (HPLC).Results There were good specificities of the TLC method to identify Rhei Radix et Rhizoma,Bupleuri Radix,Forsythiae Fructus,Puerariae Lobatae Radix.The linear range of baicalin was 0.116 2-1.743 0 μg (r =1.000 0).The average of recovery and RSD were 100.61％,0.79％ (n =6),respectively.Conclusion The method established in this study is simple,accurate,reliable and suitable to be applied to quality control for the preparation of Xiao' er resuqing granules.

A case of arrhythmias syncope diagnosed by implantable loop recorder / 中南大学学报(医学版)

Syncope is a clinical symptom for many kinds of diseases.The reasons for some syncope are still not clear even after a comprehensive and systematic examination,known as unexplained syncope.The clinical data of one patient with recurrent syncope,who had received the implanted loop recorder (ILR) in Xiangya Hospital,Central South University,were retrospectively analyzed.The instrument recorded the first syncope at a time of recurrence for the sustained ventricular tachycardia in 704 days after ILR.The patient was thus diagnosed as arrhythmic syncope and received an operation with implantable cardioverter defibrillator.The ILR is a new type of examination device for patients suffering from syncope with suspected cardiac rhythm due to its long monitoring time,low infection rate and high safety.It possesses high clinical value in the diagnosis of patients with arrhythmic syncope.

HPLC Fingerprint Analysis of Wuzi Yanzong Pills / 医药导报

Objective To establish the high performance liquid chromatography(HPLC) fingerprint of wuzi yanzong pills. Methods HPLC was performed on Agilent Extend C18 column (250 mmí4. 6 mm,5 μm) with a gradient elution system using acetonitrile:methanol (101)-0. 4% phosphoric acid as the mobile phase. The column temperature was set at 30 ℃ and the flow rate was 1. 0 mL·min-1 . The eluate was detected at the wavelength of 254 nm. Chromatographic peaks were identified by LC-MS method. Results Nine common peaks in wuzi yanzong pill samples were identified by comparing their LC-MS data with those of reference compounds and related reference reports. The HPLC fingerprint of wuzi yanzong pills was finally developed based on the analysis of sixteen batches of samples and their similarities were above 0. 93. Conclusion This method has high precision,stability and repeatability. This study could be used for overall quality assessment of wuzi yanzong pills.

Biosynthesis of amorpha-4,11-diene, a precursor of the antimalarial agent artemisinin, in Escherichia coli through introducing mevalonate pathway / 生物工程学报

Artemisinin-based combination therapies (ACTs) are recommended to be the most effective therapies for the first-line treatment of uncomplicated falciparum malaria. However, artemisinin is often in short supply and unaffordable to most malaria patients, which limits the wide use of ACTs. Production of amorpha-4,11-diene, an artemisinin precursor, was investigated by engineering a heterologous isoprenoid biosynthetic pathway in Escherichia coli. The production of amorpha-4,11-diene was achieved by expression of a synthetic amorpha-4,11-diene synthase gene in Escherichia coli DHGT7 and further improved by about 13.3 fold through introducing the mevalonate pathway from Enterococcus faecalis. After eliminating three pathway bottlenecks including amorpha-4,11-diene synthase, HMG-CoA reducase and mevalonate kinase by optimizing the metabolic flux, the yield of amorpha-4,11-diene was increased by nearly 7.2 fold and reached at 235 mg/L in shaking flask culture. In conclusion, an engineered Escherichia coli was constructed for high-level production of amorpha-4,11-diene.

EFFECT OF ORGANIC SUBSTANCE ON THE GROWTH OF PORPHYRIDIUM CRUENTUM / 微生物学通报

The growth of P. cruentum when added organic carbon source, organic nitrogen source and group B vitamin into medium were investigated in the present work. Results showed that glucose promoted growth rate observably. When addedZ% (W/V)glucose into the medium, the growth rate was doubled and biomass increased 92.6%to that of control after incubated 10 days . organic nitrogen source restrained the growth or harmed to P. cruentum. Vitamin Be and B12 also promoted the growth rate.

Studies on separation of extracellular polysaccharide from Porphyridium cruentum and its anti-HBV activity in vitro / 中国海洋药物

Objective To investigate the separation of extracellular polysaccharide from Porphyridium cruentum and its anti-HBV activity in vitro.Methods The extracellular polysaccharide(EP) of Porphyridium cruentum was extracted and isolated from the culture medium.HBeAg was assayed by ELISA and MTT methods.Results Element analysis showed that the extracellular polysaccharide contained N 0.82%,C 32.91%,H 6.19% and amino acid(2.49%).The IR and UV spectrum of EP determination showed typical absorption curve of polysaccharide,pyranglycoside linkage,sulfate ester.EP had a significant inhibition to HBeAg excretion with low toxicity on the cells and a better therapeutic index(TI).Conclusion The results suggested that EP has inhibitory effect on the hepatitis B virus in vitro.

Research on the hypoglycemic effection of marine alga Porphyridium cruentum in experimental diabetic mice / 中国海洋药物

Objective To investigate the hypoglycemic and antioxidation effects of the red alga Porphyridium cruentum in diabetic mice induced by alloxan. Methods The diabetic models were established by intravenous administration of alloxan 70 mg?kg~(-1).Then the diabetic mice randomly were divided into four groups, Among them ,two groups were given ig the algal biomass 600,1200mg?kg~(-1) ,respectively. After 18 days, the contents of blood glucose, plasma lipids and the activities of SOD were detected. Results Compared with the diabetic model, the algal biomass 600,1200mg?kg~(-1) could significantly decrease the blood glucose.A trend towards lower plasma triglyceride level was observed in test groups. In addition the activity of plasma SOD was significantly enhanced in high dose group. Conclusion Porphyridium cruentum could reduce the level of blood glucose and effectively enhance the ability of antioxidation in the diabetic mice induced by alloxan.

Nutritional evaluation of porphyra haitanensis / 中国海洋药物

The main nutrient compositions of dry Porphyra haitanensis cultivated in Lianjiang,Fujian Province were analyzed: the total amino acids content was 35.48%. The scores of amino acids were 71 for Ile and 80 for Leu. Crude fatty acids was as low as 0. 5%,in which EPA took 24.8%. Minerals in Porphyra haitanensis were abundant. The content of Ca、Fe、 Zn、Mg were 3. 63、0. 56、0. 095 and 3. 29mg?g～-1. The results indicated that Porphyra haitanensis is a kind of health food with high nutrition.

EFFECT OF ORGANIC SUBSTANCE ON THE GROWTH OF PORPHYRIDIUM CRUENTUM / 微生物学通报

The growth of P.cruentum when added organic carbon source. organic nitrogen source and group B vitamin into medium were investigated in the present work. Results showed that glucose promoted growth rate observably. When added2%(W/V)glucose into the medium, the growth rate was doubled and biomass increased 92 6%to that of control after incubated 10 days .organic nitrogen source restrained the growth or harmed to P.cruentum. Vitamin B 2 and B 12 also promoted the growth rate.

Determination of ?-Pinene in Folium Pini by high performance capillary gas chromatography / 中成药

Objective: To establish a new method for determination of ? pinene in Folium Pini. Method: The contents of ? pinene were determined by high performance capillary gas chromatography with sequential increase of temperature programming. Results: The calibration curve showed good linearity over the range of 0.424 2.12?g( r =0.9999). The average recovery was 97.9% and the relative standard deviation was 2.9%( n =5), and it was found that the contents of ? pinene in dry herbs were markedly different from the contents in fresh herbs. Conclusion: The method is simple, and accurate with a good reproducibility and can be applied as quantitative analysis method for Folium Pini.

Determination of guaiacul in Succus Bambusae by HPCG / 中成药

Objective: To establish a new method for determination of guaiacul in Succus Bambusa. Methods: The contents of guaiacul were determined by HPCG with sequential increase of temperature on a HP 6890 gas chromatograph. Results: The calibration curve showcd good linearity over the range of 0 ng～47.808 ng(r=0.99995). The average recovery was 99.64% and the relative standard deviation was 2.1%(n=6). Conclusion: The method is simple, and accurate with a good reproducibility and can be used as quantitative analysis method for Succus Bambusae.