Effect of Siji Kangbingdu Mixture on Inhibition of the Proliferation of Coxsackievirus A16 / 医药导报

Objective To verify the antiviral effects of Siji Kangbingdu mixture (SJKBDM) against coxsackievirus A16 (CoxA16).Methods Vero cells and 5-day-old suckling mice, injected with 75/50 and 1×106 TCID50 CoxA16, were used as evaluation models.The preventive influences of SJKBDM against CoxA16 in Vero cells were assessed in the models.The effects of SJKBDM on the mortality, survival time, change rate of body weight, and clinical symptom scores of suckling mice were observed.Results ①The half maximal inhibitory concentration of SJKBDM on Vero cells was 9.59 mg·mL-1.②Toxic effects were not observed from 32.3 g·kg-1 single dose or continuous intraperitoneal injectin of SJKBDM in suckling BALB/c mice.③The SJKBDM had significant inhibitory effect against CoxA16 virus.Doses higher than 1.22 mg·mL-1 could significantly improve the Vero cell survival rate, and the SJKBDM inhibition of 75/50 TCID50 CoxA16 induced pathological changes in Vero cells.④The SJKBDM significantly improved clinical symptoms of mice with CoxA16 viral infection, especially with crude drug doses of higher than 1.62 g·kg-1.The survival rate and other indicators were comparable or slightly higher compared with ribavirin, and the clinical score was higher than that of ribavirin.Conclusion The SJKBDM has significant inhibitory effect on CoxA16 cell proliferation, significantly decreases death rate, and improves clinical symptoms of mice infected with CoxA16 virus.

Plasma ET,NO,CGRP and EGF detection after the acupuncture at Zusanli point in patients with chronic atrophic gastritis / 国际检验医学杂志

Objective To study the clinical significance of plasma ET ,NO ,CGRP and EGF detection after acupuncture at Zusanli pointinpatientswithchronicatrophicgastritis.Methods 36casesofpatientswithchronicatrophicgastritiswereenrolledinthe study ,whose plasma ET ,CGRP ,EGF were measured by using radio immunoassay and NO by using biochemical methods .Results After acupuncture administrated in patients with chronic atrophic gastritis ,whose plasma ET ,EGF significantly decreased ,while CGRP ,NO increased(P<0 .05) .The levels of the 4 test items were significantly different between acupuncture for 7 d and 1 d(P<0 .05) .Conclusion Acupuncture at zusanli point could lead to the regulation of the plasma ET ,CGRP ,NO and EGF ,which has time‐dose effect .