Effect of Naoling decoction on the expression of microglia and IL-6 in hippocampal CA3 region of rats with synthetic Alzheimer's disease / 中南大学学报(医学版)

OBJECTIVE@#To observe the effect of Naoling decoction on the learning and memory behaviors and the expression of microglia and IL-6 in hippocampal CA3 region of rats with Alzheimer's disease (AD), and to elucidate the potential mechanism.@*METHODS@#Thirty SD rats were randomly divided into 5 groups: a normal group, a sham-operation group, an AD group, a Naoling decoction group and a Naofukang group. The spatial learning and memory behaviors of the rats were investigated by water maze and Y-maze. The Alzheimer's disease model was established by injecting Aβ1-42 into the hippocamal of the rats. Expression of OX-42 (one of the microglia specific markers) and IL-6 in the CA3 region of hippocamal was measured by immunohistochemical stain.@*RESULTS@#Morris water maze experiment showed that the escape latency of hidden platform in the AD group was significantly delayed (P<0.05) and the average times of passing was decreased (P<0.05). Y-maze test showed that the times needed to the learn how to evade the electrical stimulation in the AD group was most than in other groups (P<0.05). Compared with the AD group, the Morris water maze test and Y-maze test of the Naoling decoction group were significantly different (P<0.05). The expression of OX-42 and IL-6 in the CA3 region of hippocamal in the Naoling decoction group was decreased (P<0.05).@*CONCLUSION@#Naoling decoction can improve learning and memory, and weaken the expression of OX-42 and IL-6 in hippocampal CA3 of AD rats, which may partly be the therapeutic mechanism of Naoling decoction for AD.

Effect of Naoling decoction on iNOS expression in hippocampal CA3 region in rats with synthetic Alzheimer's disease / 中南大学学报(医学版)

Objective To observe the effect of Naoling decoction on the expression of iNOS in hippocamal CA3 region in rats with Alzheimer's disease (AD), and to explore the potential mechanism. Methods Forty SD rats were classified into 5 groups: a normal group, a sham operated group, an AD group, a Naoling decoction group, and a Naofukang group. The Alzheimer's disease model was established by injecting Aβ1-42 into the hippocamal in the rats. Expression of iNOS in the CA3 region was measured with immunohistochemical staining. Results Compared with the normal group, the expression of iNOS protein in the hippocampal CA3 fields in the AD group increased (P<0.05). Naoling decoction decreased the iNOS expression. Conclusion Naoling decoction can significantly reduce the expression of iNOS in the AD model rats, suggesting that Naoling decoction can be used for AD rats.

Effect of Naoling decoction on the expression of APP in hippocampal CA3 region in rats with synthetic Alzheimer's disease / 中南大学学报(医学版)

Objective To observe the effect of Naoling decoction on hippocamal histomorphology and the expression of amyloid precursor protein (APP) in CA3 region in rats with Alzheimer's disease (AD), and to explore the therapeutical and the potential mechanism. Methods Forty SD rats were classified into 5 groups: a normal group, a sham-operated group, an AD group, a Naoling decoction group, and a Naofukang group. Alzheimer's disease model was established by Aβ1-42 injected into the hippocamal in the rats. The faculty of learning and memory was evaluated by Morris water maze. The changes of cell morphology were detected by HE staining. Expression of APP in CA3 region was mea-sured with immunohistochemical staining. Results Morris water maze experiment showed that the escape latency of hidden platform in the AD group were delayed significantly (P＜0.05) and the average times of passing was decreased (P＜0.05). In the hippocampal CA3 field of the AD rats, HE staining and immunohistochemical test showed that pyramidal cells disturbed, neurons decreased significantly and expression of APP protein increased (P＜0.05). Naoling decoction treatment improved pyramidal cellular disorders and decreased APP expression. Conclusion Naoling decoction can distinctly improve the learning and memory ability, and reduce the expression of APP in the AD model rats, suggesting that Naoling decoction can be used for the treatment of AD in rats.

Long-term Toxicity of Jiuxin Fumai Injection in Rats / 中药新药与临床药理

Objective To observe the long-term toxicity of Jiuxin Fumai Injection and to investigate the safety of clinical medication.Methods The rats were given intramuscular injection with Jiuxin Fumai Injection in large,medium,small dosage(respectively 20,10,5g? kg-1)every day for two weeks,and normal saline group served as the normal control.Two weeks after drug withdrawal,the toxic reaction in rats was observed.Results After two-week continuous administration,all the animals were alive.Some animals were vomiting and getting excited when administered the large dosage and medium dosage injection.The blood sugar elevated,the thoracic gland coefficient lowered,the hepatic cells were cloudily swollen in the animals of large dosage group.Two weeks after drug withdrawal,the above phenomenon vanished.There was no obvious toxic reaction in the small-dosage injection group.Conclusion Long-term administration of Jiuxin Fumai Injection in large dosage shows certain toxic reaction in rats.The safe dosage for intramuscular administration is less than 5 g? kg-1? d-1.