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1.
Journal of Zhejiang University. Medical sciences ; (6): 390-393, 2006.
Article Dans Chinois | WPRIM | ID: wpr-332137

Résumé

<p><b>OBJECTIVE</b>To analyze the reactive pattern and its clinical significance of ZCH-7-2D3 monoclonal antibody on leukemia cells.</p><p><b>METHODS</b>Bone marrow samples from 100 leukemia patients (male 59: female 41, 34 cases of children and 66 adults, aged 11 months - 77 year) were collected. A CD45 gating strategy and multi-parameter flow cytometry were used to analyze the leukemia cells.</p><p><b>RESULT</b>The positive rate (10/31) of 2D3 antibody on acute lymphocytic leukemia (ALL) patients was significantly lower than that (39/55) of acute myelogeneous leukemia (AML) (P <0.01). 2D3 was positive for all three cases of B/myeloid mixed lineage leukemia, but negative in 6 cases of chronic myelogeneous leukemia (CML), significantly lower than that in AML (39/55, P<0.01) patients. There was no difference between the positive rates of chronic lymphocytic leukemia (CLL, 3/5) and B lineage ALL (9/28, P = 0.2389). The antibody was not found to recognize the differentiation stages of either ALL and AML subtypes.</p><p><b>CONCLUSION</b>2D3 monoclonal antibody primarily recognizes AML cases. Further studies on cloning of gene encoding the antigen protein and its biological functions are warranted.</p>


Sujets)
Adolescent , Adulte , Sujet âgé , Enfant , Enfant d'âge préscolaire , Femelle , Humains , Nourrisson , Mâle , Adulte d'âge moyen , Anticorps monoclonaux , Allergie et immunologie , Anticorps antitumoraux , Allergie et immunologie , Antigènes néoplasiques , Allergie et immunologie , Immunophénotypage , Leucémie chronique lymphocytaire à cellules B , Allergie et immunologie , Leucémie aigüe myéloïde , Allergie et immunologie , Antigènes CD45 , Allergie et immunologie , Leucémie-lymphome lymphoblastique à précurseurs B et T , Allergie et immunologie
2.
Journal of Zhejiang University. Medical sciences ; (6): 118-120, 2004.
Article Dans Chinois | WPRIM | ID: wpr-341928

Résumé

<p><b>OBJECTIVE</b>To define the immune phenotype of colon cancer cells.</p><p><b>METHODS</b>Using a panel of 40 anti-human monoclonal antibodies (MoAbs), the cells of colon cancer HR8348 were analyzed with three-color flow cytometry after direct immunofluorescent staining.</p><p><b>RESULTS</b>HR8348 cell line did not express CD2, CD3, CD4, CD5, CD7, CD8, TCR, CD10, CD11b, CD14, CD16, CD19, CD22, CD25, CD28, SmIg, CD33, CD35, CD36, CD41a, CD45, CD45RA, CD45RO, CD56, CD61, CD64, CD66b, CD69, CD71, CD117, CD122 and P-glycoprotein but expressed CD13, CD15, CD20, CD38, CD95 and HLA-DR.</p><p><b>CONCLUSION</b>The results demonstrate that colon cancer cell line HR8348 shares some antigenic determinants with leucocyte lineage.</p>


Sujets)
Humains , Glycoprotéine P , Antigènes CD , Lignée cellulaire tumorale , Tumeurs du côlon , Chimie
3.
Chinese Journal of Pediatrics ; (12): 334-337, 2003.
Article Dans Chinois | WPRIM | ID: wpr-345497

Résumé

<p><b>OBJECTIVE</b>Acute leukemia (AL) is one of the most common malignant diseases in children. AL immunophenotypes are known to be benefit to the predictable prognoses and specific therapy. Recently, the accuracy of AL immunophenotyping was dramatically improved with the application of the flow cytometry, the new monoclonal antibodies, the improvement of the gating strategies and the multi-parameter analytic techniques. The aim of this study was to evaluate the value of multi-color flow cytometry in the immunophenotyping of acute leukemia in children.</p><p><b>METHODS</b>Three- or four-color flow cytometry and CD(45)/Side Scatter (SSC) gating were used to analyze the surface and cytoplasmic (Cy) antigen expressions in 222 successive cases of childhood acute leukemia.</p><p><b>RESULTS</b>Cells from 222 cases of children with acute leukemia were analyzed. Based on the diagnostic criterion proposed by European Group for the Immunological Characterization of Leukemia (EGIL), four categories could be identified: the undifferentiated type accounted for 0.9%, acute myeloid leukemia (AML) 35.1%, acute lymphoblastic leukemia (ALL) 55.9%, and mixed lineage AL 8.1%. Of 124 patients with ALL, 94 patients (75.8%) were classified as B lineage and 30 patients (24.2%) T lineage ALL. Antigen aberrant expressions were found in AML (24.4%), B lineage ALL (36.2%) and T lineage ALL (30.0%). CD(7) was the most commonly expressed lymphoid antigen in AML (12.8%), followed by CD(19) (6.4%) and CD(2) (5.1%). CD(13) was the most commonly expressed myeloid antigen in ALL (18.5%), followed by CD(15) (11.3%), CD(11b) (6.5%) and CD(33) (4.3%). CD(117) and CD(56) presented in 73.3% and 38.0% cases of AML, respectively, but were generally absent on blast cells of ALL. CyCD(22), CyCD(3) and CyMPO were specifically expressed in B lineage, T lineage and myeloid lineage leukemia, respectively, and the first two could be more sensitively detected than they were on cell membrane surface.</p><p><b>CONCLUSIONS</b>Multi-color flow cytometry is a reliable technique in the diagnosis, differential diagnosis and classification of acute leukemia in children.</p>


Sujets)
Adolescent , Enfant , Enfant d'âge préscolaire , Femelle , Humains , Nourrisson , Mâle , Maladie aigüe , Cytométrie en flux , Méthodes , Immunophénotypage , Leucémies , Classification , Diagnostic , Allergie et immunologie
4.
Chinese Journal of Hematology ; (12): 228-230, 2003.
Article Dans Chinois | WPRIM | ID: wpr-354884

Résumé

<p><b>OBJECTIVE</b>To explore the expression of CD(117) on different types of leukemia and its significance.</p><p><b>METHODS</b>CD(117) expression was analysed by three-color flow cytometry with CD(45)/SSC gating strategy.</p><p><b>RESULTS</b>CD(117) was expressed in 68% of acute myeloid leukemia (AML) and 80% of chronic myeloid leukemia in blast crisis (CML-BC) cases, but in only 2% of acute lymphoblastic leukemia cases. CD(117) was negative in CML in chronic phase and chronic lymphocytic leukemia. Cases in AML, CD(117) was expressed in 72% of M(0)/M(1), 88% of M(2), 50% of M(4), 75% of M(5a), 100% of M(6) and 100% of M(7) cases but was less expressed in M(3) (39%) and M(5b) (29%) cases. Expression of CD(117) was more frequent than CD(34) and HLA-DR in M(3) and some M(2) cases. There was a significant inverse relationship between CD(117) and CD(14) expression in AML.</p><p><b>CONCLUSIONS</b>Analysis of CD(117) expression may be of help in distinguishing myeloid from lymphoid leukemia and leukemia clone from normal cells.</p>


Sujets)
Adolescent , Adulte , Enfant , Enfant d'âge préscolaire , Femelle , Humains , Nourrisson , Mâle , Adulte d'âge moyen , Diagnostic différentiel , Cytométrie en flux , Leucémie lymphoïde , Diagnostic , Métabolisme , Leucémie myéloïde , Diagnostic , Métabolisme , Protéines proto-oncogènes c-kit
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