Marcaçäo in vitro de hemácias com tecnécio-99m: aplicaçäo clínica em cintilografia esplênica seletiva; nota técnica / \"In vitro\" labeling of red blood cells with technetium-99m: clinical application for selective splenic scintigraphy - techinical note

A cintilografia esplênica seletiva pode ser o exame de eleiçäo quando há a necessidade de se estudar especificamente o baço. Para isso, descrevemos um método simplificado para marcaçäo in vitro de hemácias com tecnécio-99m com posterior desnaturaçäo térmica. Através dessa técnica é possível obter imagem cintilográfica esplênica com um número mais reduzido de manipulaçöes, quando comparada a métodos similares

Labelling of the platyhelminth Dusegia tigrina with 99mTechnetium

The study of the labelling of planaria with 99mTc shows that the incorporation of radioactivity in this platyhelminth increases with an increase sin SnCl2 concentration from 0.13 to 1.3 micronM, reaching a plateau in the range of 1.3-130 micronM then decreasing with 1300 microng. At concentrations of 1.3 and 13 microngM SnCl2, a stronger binding of 99mTc was obtained. The biological viability of the labelled planaria was not altered when the described methodology was used. The advantage of this new labelling technique is that it is possible to obtain a platyhelminth preparation labelled with a radionuclide that is very cheap, is easily available and is a gamma emitter with a photon energy of 140 KeV