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Braz. arch. biol. technol ; 63: e20190151, 2020. tab, graf
Article Dans Anglais | LILACS | ID: biblio-1132269

Résumé

Abstract A comprehensive comparison of the main fermentation parameters, productivity, yield and final L-lactic acid concentration, obtained through batch, fed-batch and continuous cultivations using Lactobacillus casei CCDM 198 and a model cultivation medium was carried out. Using this data, a pulse-feed fed-batch process was established for testing chicken feather hydrolysate as a replacement for all complex nitrogen sources (yeast and beef extracts and peptone) in the medium. As comparably high values of productivity (about 4.0 g/L/h) and yield (about 98 %) were reached under all cultivation conditions, the maximum final L-lactic acid concentration (116.5 g/L), as achieved through pulse-feed fed-batch fermentation, was chosen as the most important criterion for process selection. Fed-batch cultivation with chicken feather hydrolysate as both a complex nitrogen source and a neutralizing agent for maintaining constant culture pH yielded half the concentration of L-lactic acid compared to the model medium. We demonstrate here that chicken feather hydrolysate has potential for use in the production of L-lactic acid but its utilization requires further optimization


Sujets)
Animaux , Acide lactique/métabolisme , Fermentation , Lacticaseibacillus casei/croissance et développement , Biotechnologie/méthodes , Chromatographie en phase liquide à haute performance , Biomasse , Bioréacteurs , Hydrolyse
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