Varieties textual research and usage investigation of white flos gentianae, a Tibetan medicine / 中国中药杂志

<p><b>OBJECTIVE</b>To clarity the original plants and the main application varieties of White Flos Gentianae.</p><p><b>METHOD</b>Herbal textual research, wild specimen collection, investigation and collection of the samples from Tibetan hospital, Tibetan pharmaceutical factory and medical material market were carried out simultaneously to identify the original plants of White Flos Gentianae.</p><p><b>RESULT</b>The results of varieties textual research and specimen identification showed that Gentiana szechenyii, G. purdomii and G. algida were in accord with the record of Tibetan herbal textual The three species above were the original plants of White Flos Gentianae. The identification of 20 batches samples showed that G. szechenyii was the main application variety. The other varieties were only used in Tibetan hospitals. All the samples above were flowering branches.</p><p><b>CONCLUSION</b>It was necessary to strengthen the research on variety systematization of White Flos Gentianae make a further discussion on the taxonomy position of G. purdomii, G. algida and the white flos population. Its was also nessary to establish and improve the quality standard of different variety based on the principle of "one species, one name". The quality specification of White Flos Gentianae should be established and improved to standard clinical utilization and produce feeding. More study of resources investigation and cultivation of G. szechenyii should be carried on to meet the demand of produce and clinic.</p>

Mathematical modeling for selecting center locations for medical and health supplies reserve in Hainan Province

OBJECTIVE@#To explore how to choose the center locations to build the medical and health supplies reserve among many island towns.@*METHODS@#The center locations were selected from 18 towns Hainan Province, it's maximum service range (distance) was required to reach the minimum, or to minimize.@*RESULTS@#Three scenarios were considered, the center locations included only one town, two towns, three towns. By the use of graph theory and MATLAB programming, a mathematical model was established to obtain the shortest distance and the shortest path between arbitrary two towns.@*CONCLUSIONS@#We find out the center sites under certain conditions, and determine the specific service ranges of the center sites.

Establish and optimization of real-time fluorescent reverse transcription loop-mediated isothermal amplification for detection of avian influenza H5 hemagglutinin gene / 病毒学报

H5 subtype avian influenza (AIV-H5) is a major causative agent of animalloimia a rapid and sensitive molecular biological diagnosis is crucial to the control program of AIV-H5. AIV-H5 real-time fluorescent reverse transcription loop-mediated isothermal amplification (qRT-LAMP) was established by means of heat treatment of the samples. The sensitivity, specificity and repeatability of this method were assessed and the performance of Calcein,SYBR Green I,HNB,SYTO 81 in colorimetric detection was comparatively analyzed to screen the optimum dye. The results showed the sensitivity of this method was 100 times higher than that of standard real-time fluorescent RT-PCR, and the detection limit was one copy of the gene per reaction. This method had no cross-reactivity with other common avian respiratory tract infectious disease-related pathogens such as IBV and NDV. The present study suggested Calcein was the optimum dye. Small-scale tests suggested this method was reliable for survey monitoring of AIV-H5 on the spot, indicating its potential applications in field investigation.