Résumé
Objective To investigate the inhibition of Jin’s hemorrhoid ointment extract on proliflation of human umbilical vein endothelial cell (ECV-304), then to study the mechanism of treating hemorroid. Methods MTT assay was used to observe the inhibition of various concentrations Jin’s hemorrhoid ointment extract and single Chinese Herbs of compound on human umbilical vein endothelial cell (ECV-304). Results Various concentrations Jin’s hemorrhoid ointment extracts inhibited proliflation of human umbilical vein endothelial cell, which depended on concentration. All of Rheum decoctions and high concetration Curcuma decoction inhibited ECV-304 proliflation. Conclusion Jin’s hemorrhoid ointment can inhibit proliflation of human umbilical vein endothelial cell, and Rheum maybe the main effective part of Jin’s hemorrhoid ointment, Curcuma also take part in it.
Résumé
Objective To investigate the effect of apoptosis with portal vein occlusion in various degrees or times and the following restoration portal vein flow on intestinal mucosal cells,to analyze its possible pathogenesis. Methods 40 healthy adults white Japanese rabbits were randomly divided into one controlled group and four experimental groups (according to portal vein half-clamping for 30 min, 45 min and portal vein full-clamping for 30 min, 45 min, divided into B1、C1、B2、C2 groups.).Each experimental group with portal vein occlusion on certain times then with restoration of portal vein flow for 2h. At choiced time rabbit guts were continuously cut to sections for HE、TUNEL staining and Bcl-2、Bax protein immunohistochemical staining,to observe the injury apoptosis in intestinal mucosa and the relationship between the expressions of Bcl-2 and Bax protein. Results Compared with control group A, down-regulation of Bcl-2 expression, up-regulation of Bax expression and the index of apoptotic cell increased were found in each experimental group. there exist significantly differences between each groups for Bcl-2 expression (P