Résumé
Objective: To use three dimensional model in vitro to mimic solid tumor growth in vivo and explore the relationship between the multicellular resistance of colon carcinoma cell and the over-expression of cell adhesion molecules (integrin α V and integrin β3). Methods: The colon carcinoma HT-29 multicellular spheroids (MCS) model were constructed with three dimensional cell culture methods. The mRNA and protein expressions of integrin α V and integrin β3 was detected by RT-PCR and immunofluorescence analysis and compared between monolayer cells (MC) and MCS. The viability and apoptotic rate of MC and MCS were detected by flow cytometry (FCM) after treatment with 5-fluorouracil (5-FU). Results: The expression levels of integrin α V and integrin β3 in three dimensional cell culture model of HT29 MCS were much higher than those in MC (P < 0.01). Compared with MC, the sensitivity of MCS to the anticancer drug 5-FU significantly decreased ( P < 0.05), and the rate of 5-FU-induced apoptosis significantly decreased (0.346 ± 0.035 vs 0.235 ± 0.024, P < 0.05). Conclusion: Over-expression of cell adhesion molecules integrin α V and integrin β3 may increase the multi-cellular resistance of colon carcinoma cells.