A brief introduction of identification of tumor antigen-specific TCR gene / 中国免疫学杂志

Adoptive therapy with T cells modified by tumor antigen specific T cell receptor(TCR)gene become a research hotspot in tumor biotherapy.The generation of high-affinity TCR is a significant pre-condition for TCR-T therapy.In this review,we focus on the identification of tumor antigen-specific TCR genes,which based on RT-PCR,single cell RT-PCR and CDR3 length polymorphism analysis.

Application of CRISPR/Cas9 in tumor immunotherapy / 中国免疫学杂志

In recent years,tumor immunotherapy has attracted more and more attention because of its remarkable curative effect and innovation.With the development of CRISPR gene editing system and its application in many fields,researchers have seen its great potential,especially in the field of cancer research.For adoptive cell therapy,CRISPR/Cas9 can knockout endogenous TCR and HLA-Ⅰ genes in TCR-T and CAR-T cells to generate universal effective T cells.It provided a new method to inhibit and block the immune checkpoint.The application of CRISPR/Cas9 in genetic screening makes antibody targeted therapy to a new level.CRISPR has greatly promoted the research of tumor immunotherapy.This article introduced the application of CRISPR/Cas9 gene editing system in tumor immunotherapy.