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Chinese Journal of Medical Genetics ; (6): 52-55, 2004.
Article Dans Chinois | WPRIM | ID: wpr-329400

Résumé

<p><b>OBJECTIVE</b>To construct a phosphatidylinositol 4-kinase beta (PI4K-beta) mutant with the 325th to 373rd amino acid codons deleted, and try to develop a simple method for constructing middle fragment deletion mutant.</p><p><b>METHODS</b>In line with the mechanism of gene splicing by overlap extension(SOE), an additional PCR was used to get the PI4K-beta mutant in which the 325th to 373rd amino acid codons were deleted. Then the mutated gene was cloned into pCMV-Tag4A mammalian expression vector.</p><p><b>RESULTS</b>A mutant with the 325th to 373rd amino acid codons deleted was constructed successfully.</p><p><b>CONCLUSION</b>The improved SOE is a very effective and reliable method to construct middle fragment deletion mutant. It is worthy to be popularized.</p>


Sujets)
1-Phosphatidylinositol 4-kinase , Génétique , Séquence nucléotidique , Vecteurs génétiques , Génétique , Données de séquences moléculaires , Mutagenèse dirigée , Mutation , Génétique , Réaction de polymérisation en chaîne , Méthodes , Ingénierie des protéines , Méthodes , Protéines recombinantes , Génétique , Délétion de séquence
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