Résumé
<p><b>OBJECTIVE</b>To detect the expression of Per2 in non-small cell lung cancer (NSCLC), and analyze its clinical significance.</p><p><b>METHODS</b>The expression of Per2 was determined in 60 NSCLC and 20 normal lung tissues by immunohistochemical assay, and the relationship between Per2 expression and clinicopathological features was analyzed.</p><p><b>RESULTS</b>The positive expression rates of Per2 in NSCLC and normal lung tissues were 71.7% and 95.0%, respectively (P < 0.05). The expression of Per2 in NSCLC was correlated with pathological differentiation and TNM stage (P < 0.05).</p><p><b>CONCLUSION</b>The expression of Per2 in NSCLC is decreased. The negative expression of Per2 may contribute to the development and invasion in NSCLC.</p>
Sujets)
Adulte , Sujet âgé , Femelle , Humains , Mâle , Adulte d'âge moyen , Carcinome pulmonaire non à petites cellules , Métabolisme , Anatomopathologie , Chirurgie générale , Régulation de l'expression des gènes tumoraux , Tumeurs du poumon , Métabolisme , Anatomopathologie , Chirurgie générale , Métastase lymphatique , Grading des tumeurs , Stadification tumorale , Protéines circadiennes Period , Métabolisme , FumerRésumé
<p><b>OBJECTIVE</b>To establish a reverse remodeling heart model in rats and observe collagen and TGF-β expression and relevant microRNAs changes during reverse remodeling.</p><p><b>METHODS</b>Lewis rats were divided into four groups including sham (NL, n = 10), abdominal aortic constriction (AAC, n = 10), heterotopic transplantation of abdominal aortic constriction (AAC-HT, n = 9) and heterotopic transplantation of normal heart (HT, n = 8). Left ventricular wall thickness and LV cavity were measured by echocardiography. The cardiomyocyte cross-sectional area (CSA) was determined on HE stained sections. Immunohistochemical and qRT-PCR were used to detect collagen and TGF-β expressions. miRNAs were detected by MicroRNA microarray.</p><p><b>RESULTS</b>Heart weight, left ventricular wall thickness and CSA were significantly increased in AAC hearts compared to those in the NL and AAC-HT hearts. The collagen and TGF-β were increased in AAC hearts and further increased in AAC-HT hearts. miRNA microarray evidenced more than two folds changes on 82 miRNAs compared to NL (10 in AAC, 32 in AAC-HT and 40 in HT).</p><p><b>CONCLUSION</b>Rat abdominal aortic constriction and heterotopic transplantation could be used as a reverse remodeling heart model and significant collagen and TGF-β as well microRNA expression changes were evidenced in this model.</p>