Résumé
The wall of rat anterior venae cavae has cardiomyocytes running in different directions. These cardiomyocytes were connected by the intercalated discs. Some myocytes in the inner layer of the AVC near the atrium contained specific granules, which were very similar to those in the atrial myocytes in shape and distribution, but the number of the specific granules in the myocytes of venae was much less than that in the myocytes of atrium. Well-developed Golgi complexes, rough surfaced endoplasmic reticulum and multivesicular bodies were frequently found near the specific granules. Immuno-electron microscopy of the specific granules of the venae by using rabbit anti-synthetic rat ANP (1-28) serum, postembedding method and immunogold staining disclosed gold particles on the specific granules of myocytes in the venae were found in the atrial specific granules. It was also shown that the diameter of the labelled granules was somewhat related to the number of the labelling gold particles. No gold particles of the myocytes of the venae when the section was beforehand treated with the solution containing a combination of rat ANP and rabbit anti-ANP serum. The result indicated the presence of atrial natriuretic peptide (ANP) in the specific granules of myocytes of rat anterior venae cavae as well as in those of the atrium. The formation process of the specific granules and the possible way of the release of their contents were discussed.
Résumé
By morpometric and immunohistochemical methods, it is observed that the volume density, the numerical density on area and the numerical density of specific granules in the perinuclear region of the atrial myocytes in the aging rats ars significantly less than that in the young groups (p
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Objective To demonstrate in vivo that there is different degree myocardium apoptosis during acute ischemia and reperfusion and to explore prelimarily the relations between myocardium apoptosis and the expression of Bcl 2/Bax protein in rats. Methods The morphologic features of apoptotic cardiocytes were pursuited by transmission electron microscopy.DNA extracted from cardiocytes were used to identify the apoptotic DNA ladder by agarose gel eletrophoresis. DNA fragmentation was identified histochemically by TUNEL staining. Expression of Bcl 2/Bax protein in apoptotic myocardium was observed by means of immunohistochemistry and analysed by image analysis system. Results In both ischemia and reperfusion groups morphologic features of apoptotic myocardium were observed by transmission electron microscopic and the apoptotic cardiocytes were detected by the TdT mediated dUTP nick end labeling (TUNEL) staining. However, the apoptotic DNA ladder was observed only in the 2 hour reperfusion group. The reperfusion group showed a decrease in OD value of the Bcl 2 protein( P
Résumé
The kidneys removed from six female adult dogs were injected through the renal artery with ABS at a constant pressure between 33.3—33.7 kPa(250—280mmHg). Small specimens cut out from the kindneys were digested in 3％ trypsin solution for 48 hours and then eroded in 50％ HCl. The cast specimens were studied under the scanning electron microscope. The results were outlined as follows: 1. The corrosion-cast models of the renal glomerulus of the dogs observed under the SEM could be divided into three kinds: the spherical glomerulus, the chrysanthemum-like glomerulus and mixed glomerulus. 2. In general, the afferent and efferent arteriolae entered or exited at the hilum of glomerulus. Rarely, the efferent vessel left the glomerulus at the opposite side of the hilum. Anastomoses between the afferent and efferent or between the efferents vessels were found in our casts. 3. The capillary network of the juxtaglomerular apparatus was observed at the hilum of glomerulus in our casts. The diameters of the meshes of the capillary network were smaller than that of the vessels themselves.