Résumé
The mitochondrial cytochrome c oxidase subunit I (CO I) gene and the second internal transcribed spacer region (ITS II) gene of Opisthorchis viverrini were compared among O. viverrini from various areas in northeast Thailand. The nucleotide sequences of partial CO I gene (417 bp) of O. viverrini differed among O. viverrini originated from Ubon Ratana, Leongpleuy, Ban Phai, Maha Sarakham, and Chatturat. These intraspecific variations were classified into 5 patterns but no area-specific pattem was observed. Amino acid sequence deduced from the nucleotide sequences of these genes was identical. Nucleotide sequences of a region of the O. viverrini ITS II gene (296 bp) from different areas were identical. However, they were different from those of Clonorchis sinensis, Haplorchis taichui, H. pumilio, Fasciola gigantica, Echinostoma malayanum and Centrocestus sp..
Sujets)
Séquence d'acides aminés , Animaux , Séquence nucléotidique , Cricetinae , ADN des helminthes/composition chimique , ADN mitochondrial/composition chimique , Espaceur de l'ADN ribosomique/composition chimique , Complexe IV de la chaîne respiratoire/composition chimique , Humains , Données de séquences moléculaires , Opisthorchiase/parasitologie , Opisthorchis/enzymologie , Réaction de polymérisation en chaîne , Alignement de séquences , Similitude de séquences d'acides nucléiques , Spécificité d'espèce , ThaïlandeRésumé
Two gravid strobila without scolex were passed by a 23-year-old male in Mie Prefecture, Japan. Morphological features were comparable to characteristics of Bertiella studeri. Although two children's cases have already been reported, this is the first case of an adult in Japan since the occurrence of B. studeri was proven in Japan.