Résumé
Objective to investigate the clinical diagnosis of female hypertension with chest pain.Methods The clinical coronary angiography data of 77 female cases of hypertension with chest pain diagnosed as coronary heart diseased (CHD) and angina pectoris were analysed retrospectively.The comparison of the risk factors and clinical features in the two groups was made.Results Clinically,it was difficult to differentiate hypertension with chest pain and coronary heart failure,but dynamic EDG and typical angina pectoris suggested the possibility of coronary heart disease.Conclusion Coronary angiography is still essential to diagnose CHD.
Résumé
AIM: To investigate the effect of antisense RNA on osteopontin (OPN) expression in renal tubular epithelial cells. METHODS: Cell clone expressing stably OPN antisense RNA was formed by transfering retroviral vector expressing OPN antisense RNA into renal tubular epithelial cells, NRK52E cells, using liposome, with cell clones transfected by empty vector and vector expressing OPN sense RNA as controls. Ribonuclease protection assay(RPA), Western Blot, ELISA and assay of OPN activity were performed to detect expression of OPN mRNA and protein in above clones cultured with or without epidermal growth factor(EGF). RESULTS: The antisense RNA was only expressed by antisense clone. Antisense clone, sense clone and empty clone all expressed OPN mRNA. EGF enhanced expression of OPN mRNA, but not OPN antisense RNA or OPN sense RNA in above clones. OPN protein was not expressed in antisense clone cultured with or without EGF and empty clone cultured without EGF, but was expressed in sense clone cultured with or without EGF and empty clone cultured with EGF. CONCLUSION: Antisense RNA can inhibit OPN protein expression by means of preventing OPN mRNA translation, but not inhibit OPN mRNA transcription in renal tubular epithelial cells.