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1.
Chinese Journal of Natural Medicines (English Ed.) ; (6): 847-854, 2017.
Article Dans Anglais | WPRIM | ID: wpr-812050

Résumé

The study aimed to investigate the intervening role of Didang decoction (DDD) at different times in macrovascular endothelial defense function, focusing on its effects on the AMP-activated protein kinase (AMPK) signaling pathway. The effects of DDD on mitochondrial energy metabolism were also investigated in rat aortic endothelial cells (RAECs). Type 2 diabetes were induced in rats by streptozotocin (STZ) combined with high fat diet. Rats were randomly divided into non-intervention group, metformin group, simvastatin group, and early-, middle-, late-stage DDD groups. Normal rats were used as control. All the rats received 12 weeks of intervention or control treatment. Western blots were used to detect the expression of AMP-activated protein kinase α1 (AMPKα1) and peroxisome proliferator-activated receptor 1α (PGC-1α). Changes in the intracellular AMP and ATP levels were detected with ELISA. Real-time-PCR was used to detect the mRNA level of caspase-3, endothelial nitric oxide synthase (eNOS), and Bcl-2. Compared to the diabetic non-intervention group, a significant increase in the expression of AMPKα1 and PGC-1α were observed in the early-stage, middle-stage DDD groups and simvastatin group (P < 0.05). The levels of Bcl-2, eNOS, and ATP were significantly increased (P < 0.05), while the level of AMP and caspase-3 were decreased (P < 0.05) in the early-stage DDD group and simvastatin group. Early intervention with DDD enhances mitochondrial energy metabolism by regulating the AMPK signaling pathway and therefore may play a role in strengthening the defense function of large vascular endothelial cells and postpone the development of macrovascular diseases in diabetes.


Sujets)
Animaux , AMP-Activated Protein Kinases , Métabolisme , Adénosine triphosphate , Métabolisme , Aorte , Métabolisme , Maladies cardiovasculaires , Métabolisme , Caspase-3 , Métabolisme , Diabète expérimental , Traitement médicamenteux , Métabolisme , Diabète de type 2 , Traitement médicamenteux , Métabolisme , Diptera , Médicaments issus de plantes chinoises , Pharmacologie , Utilisations thérapeutiques , Cellules endothéliales , Métabolisme , Endothélium vasculaire , Métabolisme , Métabolisme énergétique , Sangsues , Mitochondries , Métabolisme , Nitric oxide synthase type III , Métabolisme , Coactivateur 1-alpha du récepteur gamma activé par les proliférateurs de peroxysomes , Métabolisme , Phytothérapie , Protéines proto-oncogènes c-bcl-2 , Métabolisme , Prunus persica , Rat Sprague-Dawley , Rheum , Transduction du signal
2.
Chinese journal of integrative medicine ; (12): 837-845, 2012.
Article Dans Anglais | WPRIM | ID: wpr-347104

Résumé

<p><b>OBJECTIVE</b>To investigate the protective effects of sodium tanshinone B (STB) on brain damage following focal ischemia-reperfusion (I/R) injury through interfering with N-methyl-D-aspartic acid receptor (NMDAR) and excitatory and inhibitory amino acids, and evaluate the potential mechanisms of the neuroprotective activity of STB.</p><p><b>METHODS</b>Transient forebrain ischemia was induced by middle cerebral artery occlusion (MCAO). The rats were randomized into a sham operated group, a model group (I/R) and three STB different dose groups. Rats were pretreated with STB at the doses of 4, 8, 16 mg/kg (STB(1), STB(2), STB(3)) for 3 days before MCAO. The expression of NMDAR1 was detected by immunohistochemistry and Western blotting. The concentrations of glutamate and γ-aminobutyric acid (GABA) were analyzed using high performance liquid chromatography.</p><p><b>RESULTS</b>STB treatment reduced neurological defect scores, cerebral infarction volume and brain water content. The levels of NMDAR1 were significantly higher in the l/R and STB(1) groups than that of the sham and the STB(3) groups (P<0.01). Optical density of NMDAR1 was significantly increased in cornu ammonis (CA)1 region of the l/R group (P<0.05). STB treatment reduced NMDAR1 optical density in the CA1 region (P<0.01). The levels of glutamate were significantly lower in the hippocampus in the STB(3) group than that of the l/R, STB(1) and STB(2) groups (P<0.01).</p><p><b>CONCLUSION</b>Preconditioning with STB appears to be a simple and promising strategy to reduce or even prevent cerebral l/R injury and has potential for future clinical application.</p>


Sujets)
Animaux , Rats , Encéphalopathie ischémique , Anatomopathologie , Cytoprotection , Modèles animaux de maladie humaine , Abiétanes , Pharmacologie , Évaluation préclinique de médicament , Médicaments issus de plantes chinoises , Pharmacologie , Hippocampe , Anatomopathologie , Modèles biologiques , Neurones , Anatomopathologie , Physiologie , Neuroprotecteurs , Pharmacologie , Répartition aléatoire , Lésion d'ischémie-reperfusion , Anatomopathologie , Résultat thérapeutique
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