Résumé
Objective:To establish a HPLC method for determinating 9 components simultaneously in Swertia chirayita. Methods:By useing water Sunfire C18 column (4.6 mm× 250 mm,5 μm); Gradient elution was carried out with methanol-0.05% phosphoric acid solution as mobile phase. Setting the column temperature at 30 ℃, the flow rate at 1.0 ml/min, and the detection wavelength at 254 nm.Results:9 components showed good linear relationship within the injection quality range. The recovery rates of wertiamarin, Gentiopicroside, Angelica glycosides,Mangiferin, Isolysine, Gentianoside, Diol glycoside, 8-hydroxy-1,3,5 trimethoxyketone, and Daisy leaf gentinone were 95.38%, 92.41%, 95.14%, 91.87%, 92.24%, 92.51%, 95.08%, 91.72%, 95.74% ( n=6). Conclusion:The method is simple, efficient, sensitive, accurate, economical and practical, with repeatability and stability. It could provide reference for the quality control and comprehensive utilization of Swertia chirayita.
Résumé
Objective@#To improve the awareness of bloodborne pathogen exposure protection knowledge, reduce the contact incidence (including sharp injuries, contacting with the broken skin and mucous mem-brane contact) , and increase the self-report incidence of bloodborne pathogen exposure in the healthcare work-ers of the investigated hospital after the intervention. To summarize effective prevention and intervention mea-sures of occupational exposure to bloodborne pathogen.@*Methods@#The project was on the basis of previous inves-tigations in a general hospital, after comparing the personnel structure and departments distribution of 727 HC-Ws before the intervention and 614 HCWs after the intervention, we selected several departments in which the healthcare workers with a higher risk of occupational exposure to bloodborne pathogens. We promoted the hospi-tal to set up occupational health prevention and control system, improved related files, supplemented by knowl-edge training, discussion and field guidance, such a comprehensive intervention in the hospital. After the inter-vention, we conducted an cross-sectional investigation, and compared the rate of protection knowledge aware-ness, self-reporting rate after exposure, occupational exposure to bloodborne pathogens (including sharp inju-ries, contacting with the broken skin and mucous membrane contact) incidence before and after the intervention.@*Results@#Before and after the intervention, the total score of the investigated population of 14 knowledge points that we focused on in training showed a significant difference (P<0.05) , total contact incidence of bloodborne pathogen exposure reduced from 81.57 persons/100 persons per year to 43.81 persons/100 persons per year, the RR of bloodborne pathogen exposure in the former investigation was 1.86 times of that in the latter investigation, the self-reported incidence increased form 2.06 persons/100persons per year to 9.45 persons/100 persons per year, self-reporting rate after exposure increased form 0.47% to 9.65%.@*Conclusion@#The interventions signifi-cantly improved the awareness of bloodborne pathogen exposure protection knowledge, effectively reduced the contact incidence of bloodborne pathogen exposure, and increased the self-reported incidence in the healthcare workers of the investigated hospital.