Studies on chemical constituents of leaves of Aquilaria sinensis / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the chemical constituents of the leaves of Aquilaria sinensis, and provide a certain of basis for the comprehensive uses of the plant of A. sinensis.</p><p><b>METHOD</b>The chemical constituents were isolated by various column chromatographic method. The structures were identified by spectral analyses of NMR, MS, et al.</p><p><b>RESULT</b>Thirteen compounds were isolated and identified as 7-hyroxy-5, 4'-dimethoxy flovone (1), 5-hydroxy-7, 4'-dimethoxy flavone (2), luteolin-7-3',4'-trimethyl (3), isocorydine (4), 4-hydroxybenzoic acid (5), triacontenoic (6), hentriacontane (7), alpha-stigmasterol (8), epifriedelanol (9), friedelan (10), friedelin (11), genkwanin (12), 5, 4'-dihyroxy-7, 3'-dimethoxy flovone (13).</p><p><b>CONCLUSION</b>Compound 4 was obtained from this genus for the first time, compounds 1, 6-11, 13 were obtained from this species for the first time.</p>

Determination of carnosic acid in rat stomach and intestine by high performance liquid chromatography method / 中国中药杂志

<p><b>OBJECTIVE</b>To establish a HPLC method to determine the carnosic acid in the stomach and intestine of rats and study its tissue distribution characteristics.</p><p><b>METHOD</b>After intragastric administration of carnosic acid (90 mg x kg(-1)), rats for each time-point were sacrificed by decapitation. After removal of the blood, various tissues were rapidly removed and weighted, all tissues were treated with a series of pretreatment before HPLC. Chromatographic separation was achieved on a Kromasil C18 column (4.6 mm x 150 mm, 5 microm) protected by an ODS guard column at 25 degrees C, using acetonitrile-0.1% phosphoric acid solution (55:45) as mobile phase, at a flow rate of 1 mL x min(-1). The wavelength of the UV detector was set at 210 nm for carnosic acid and internal standard.</p><p><b>RESULT</b>Good linearities were obtained in every tissue over a range of 0.3212-160.6 mg x L(-1). The recovery, intra-day and inter-day precision and accuracy of three concentrations of carnosic acid in tissues met the requirements of methodology. And the stability of the tissue samples were also validated. The results of distribution in stomach and intestine showed that the highest concentration was (307.1 +/- 119.2) microg x g(-1) in stomach and (33.32 +/- 17.70) microg x g(-1) in intestine after intragastric administration of carnosic acid.</p><p><b>CONCLUSION</b>The HPLC method was established to determine the concentration of carnosic acid in tissues. This method is quick, precise, and reproducible. It is the first time to study the tissue distribution of carnosic acid in rats after intragastric administration.</p>