Myosin Va is developmentally regulated and expressed in the human cerebellum from birth to old age

Myosin Va functions as a processive, actin-based motor molecule highly enriched in the nervous system, which transports and/or tethers organelles, vesicles, and mRNA and protein translation machinery. Mutation of myosin Va leads to Griscelli disease that is associated with severe neurological deficits and a short life span. Despite playing a critical role in development, the expression of myosin Va in the central nervous system throughout the human life span has not been reported. To address this issue, the cerebellar expression of myosin Va from newborns to elderly humans was studied by immunohistochemistry using an affinity-purified anti-myosin Va antibody. Myosin Va was expressed at all ages from the 10th postnatal day to the 98th year of life, in molecular, Purkinje and granular cerebellar layers. Cerebellar myosin Va expression did not differ essentially in localization or intensity from childhood to old age, except during the postnatal developmental period. Structures resembling granules and climbing fibers in Purkinje cells were deeply stained. In dentate neurons, long processes were deeply stained by anti-myosin Va, as were punctate nuclear structures. During the first postnatal year, myosin Va was differentially expressed in the external granular layer (EGL). In the EGL, proliferating prospective granule cells were not stained by anti-myosin Va antibody. In contrast, premigratory granule cells in the EGL stained moderately. Granule cells exhibiting a migratory profile in the molecular layer were also moderately stained. In conclusion, neuronal myosin Va is developmentally regulated, and appears to be required for cerebellar function from early postnatal life to senescence.

"Familias fuertes" taller de fortalecimienmto para adolescentes: satisfacción con la educación recibida / "Strong families": a workshop to strengthen adolescent families. Outcome satisfaction

Objective: This study examined family satisfaction with the education they received in the Strong Families Workshop, whose purpose is risk behavior prevention in adolescents aged 10 to 14. Patients and Method: This was a descriptive, cross-sectional study involving 100 families (parents or guardians and adolescents) from five high schools in the Metropolitan Region of Santiago, Chile. The workshop consisted of seven sessions for parents, adolescents and families. An instrument was used on participants at 6 months post intervention. It included aspects relating to the structure, process and results of the workshop. Results: 100 percent of the families attended the complete workshop. The degree of satisfaction with the family sessions in both groups was greater than 90 percent, and greater than 80 percent with respect to the schedule, frequency, quality of videos, written program material, teacher quality and content completion achievements. Conclusions: There is great interest on the part of parents and adolescents to jointly participate in family strengthening workshops. This may justify the implementation of such at the school level, considering the role of educators as promoters of health and the family as a protective factor in healthy adolescent development.

Objetivo: Este trabajo estudió la satisfacción en familias con la educación recibida en el Taller Familias Fuertes, cuyo propósito es la prevención de conductas de riesgo en adolescentes de 10 a 14 años. Pacientes y Método: Estudio descriptivo, transversal, en 100 familias (padres o tutores y adolescentes) de cinco colegios de la Región Metropolitana de Santiago de Chile. El taller, constó de siete sesiones para padres, adolescentes y familia. Se aplicó un instrumento a los participantes a los 6 meses post intervención, que consideró aspectos de la estructura, proceso y resultados del taller. Resultados: El 100 por ciento de las familias asistió a la totalidad del taller. El grado de satisfacción en ambos grupos con la sesiones en familia fue mayor al 90 por ciento y mayor al 80 por ciento respecto del horario, frecuencia, vídeos, material escrito del programa, calidad docente y logros del cumplimiento de los contenidos. Conclusiones: Existe un gran interés de padres y adolescentes en participar en forma conjunta en talleres de fortalecimiento familiar, por lo tanto se justifica ampliar su implementación a nivel escolar considerando el rol de los educadores como agentes promotores de la salud y a la familia como un factor protector del desarrollo sano del adolescente.

Tomografia computadorizada do cranio no diagnostico dos meningiomas:registro de 16 casos. / Computerized tomography of the skull in the diagnosis of meningiomas. Report of 16 cases

Os autores se referem a importancia do emprego da tomografia computadorizada do cranio para o preciso diagnostico dos meningiomas. Constam da experiencia 16 casos comprovados cirurgica e histologicamente. A media de idade foi de 52,1 anos, sendo 10 pacientes do sexo feminino e 6 masculino. Quando a localizacao, 5 eram da cavidade orbitraria e/ou asa do esfenoide, 3 parasagitais, 3 da foice, 2 supra-selares, 2 de convexidade e 1 do tentorio. Concluem tratar-se de exame de grande positividade (95-98%), praticamente inocuo e que permite, na atualidade o diagnostico precoce conduzindo, consequentemente, a melhores resultados terapeuticos.