Résumé
INTRODUCTION: Ovarian cancer is generally diagnosed at advanced stages of the disease; therefore, poor prognoses are typical. The development of tumor markers is thus of utmost importance. Prostasin is a protease that in normal tissues is highly expressed only in the prostate gland and seminal fluid. A previous study showed that prostasin is highly overexpressed in ovarian cancer cell lines. This study sought to evaluate the expression of prostasin in ovarian cancer. METHODS: Fresh tumor samples of ovarian epithelial cancer (n: 12) were analyzed for expression of prostasin mRNA (messenger ribonucleic acid) by conventional and real time quantitative PCR (polymerase chain reaction). As a standard control, a normal prostate sample was analyzed. RESULTS: Using conventional PCR, prostasin was detected in all but one sample. Using quantitative PCR, prostasin was over-expressed in all but one of the samples as compared to the control (prostate). CONCLUSIONS: These findings indicate that prostasin is overexpressed in many epithelial ovarian cancers. Further studies of prostasin as a potential biomarker for this disease are warranted.
Sujets)
Adulte , Sujet âgé , Sujet âgé de 80 ans ou plus , Femelle , Humains , Adulte d'âge moyen , Régulation de l'expression des gènes tumoraux , Dépistage de masse/méthodes , Protéines tumorales/sang , Tumeurs de l'ovaire/enzymologie , Serine endopeptidases/sang , Marqueurs biologiques tumoraux/sang , Tumeurs de l'ovaire/diagnostic , Projets pilotes , RT-PCRSujets)
Humains , Mâle , Adulte d'âge moyen , Adénocarcinome/sang , Anticorps antiphospholipides/sang , Tumeurs du poumon/sang , Tumeurs de la prostate/sang , Thrombose veineuse/sang , Adénocarcinome/immunologie , Tumeurs du poumon/immunologie , Tumeurs du poumon/secondaire , Tumeurs de la prostate/immunologie , Thrombose veineuse/immunologieRésumé
Os autores apresentaram um caso de angiolipoma epidural pela raridade dessa patologia. De localizaçao epidural torácica a lesao causava maior compressao no nível de T3-T4. Nao havia disrafismos espinhais, nem relato de uso de corticosteróides e simulava enfermidade degenerativa medular pelo tempo de evoluçao.
Sujets)
Humains , Mâle , Sujet âgé , Angiolipome , Espace épidural , Vertèbres thoraciques/chirurgie , Hypertension artérielle , Laminectomie , Équilibre postural , Signe de BabinskiRésumé
Os autores relatam um caso de um paciente de 5 meses de idade, com papiloma de plexo coróide (PPC) associado a hidrocefalia importante por super-produçao liquórica. Como decorrência do tratamento, inúmeras complicaçoes ocorreram, como hematoma subdural agudo, coleçao subdural crônica de início hipertensivo, sendo necessários vários procedimentos cirúrgicos, culminando com derivaçao ventrículo peritoneal e subduro-peritoneal.