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Gamme d'année
1.
Rev. Inst. Med. Trop. Säo Paulo ; 44(3): 121-125, 2002. tab
Article Dans Anglais | LILACS | ID: lil-314538

Résumé

A comparison of the Etest and the reference broth macrodilution susceptibility test for fluconazole, ketoconazole, itraconazole and amphotericin B was performed with 59 of Candida species isolated from the oral cavities of AIDS patients. The Etest method was performed according to the manufacturer's instructions, and the reference method was performed according to National Committee for Clinical Laboratory Standards document M27-A guidelines. Our data showed that there was a good correlation between the MICs obtained by the Etest and broth dilution methods. When only the MIC results at ± 2 dilutions for both methods were considered, the agreement rates were 90.4 percent for itraconazole, ketoconazole and amphotericin B and 84.6 percent for fluconazole of the C. albicans tested. In contrast, to the reference method, the Etest method classified as susceptible three fluconazole-resistant isolates and one itraconazole-resistant isolate, representing four very major errors. These results indicate that Etest could be considered useful for antifungal sensitivity evaluation of yeasts in clinical laboratories


Sujets)
Humains , Antifongiques , Candida , Tests de sensibilité microbienne , Fluconazole , Itraconazole
2.
Rev. Soc. Bras. Med. Trop ; 33(1): 75-78, jan.-fev. 2000. tab, graf
Article Dans Portugais | LILACS | ID: lil-301714

Résumé

Criptococose é considerada a infecçäo fúngica sistêmica oportunista mais comum em pacientes com AIDS. Nestes pacientes tem predominado como agente etiológico Cryptococcus neoformans var. neoformans, e muito raramente relata-se C. neoformans var. gattiii, mesmo nas regiöes onde se verifica a sua prevalência. Foram estudados 50 pacientes com lesöes de criptococose meningoencefálica associada com AIDS. Os isolados foram identificados através de características microscópicas e macroscópicas exibidas em meios de ágar Sabouraud, ágar niger e Christensen. As variedades de C. neoformans foram determinadas pela reaçäo de coloraçäo obtida em meio de L-canavanina glicina-azul de bromotimol (CGB). Em todos os pacientes examinados foram isolados C. neoformans, sendo identificados C. neoformans var. neoformans em 47 isolados e C. neoformans var. gattii em 3. Os resultados encontrados mostram que a criptococose em pacientes com AIDS pode também ser causada por C. neoformans var. gatti, apesar de haver predominância de C. neoformans var neoformans nesta populaçäo


Sujets)
Cryptococcus neoformans
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