Beneficial effects of autologous bone marrow mononuclear cell transplantation against ischemic bile duct in rats / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Bone marrow cell transplantation has been shown to induce angiogenesis and thus improve ischemic disease. This study evaluated the effect of bone marrow mononuclear cell (BM-MNCs) implantation on neovascularization in rats with ischemic bile duct.</p><p><b>METHODS</b>We established an animal model for ischemic biliary stenosis by clamping manipulation. There were 10 rats in each group: BM-MNCs implantation group, control group and normal group. Rat femur BM-MNCs were isolated using density gradient centrifugation. BM-MNCs or phosphate buffered saline were injected into three points around bile duct tissue in the three groups (25 µl/point). Control rats received injections of saline under similar conditions. At the 21 days after operation, cholangiography was performed. Differentiation of the engrafted cells and capillary density in the bile duct were analyzed by immunohistochemical staining.</p><p><b>RESULTS</b>Engrafted cells could differentiate into endothelial cells. The stricture rate in the implantation group was 40%, significantly lower than that in the control group (100%). The capillary density in the implantation group was significantly higher than in the control group or the normal group.</p><p><b>CONCLUSIONS</b>The implantation of BM-MNCs induced neovascularization in the ischemic bile duct. It improved the blood supply of the ischemic bile duct to prevent or decrease biliary ischemic stricture.</p>

Re-transplantation following adult-to-adult living donor liver transplantation:report of 6 cases / 中华器官移植杂志

Objective To summarize the experience and clinical characteristics in liver re- transplantation following adult-to-adult living donor liver transplantation.Methods Due to biliary complication (2 cases),vascular complication (2 cases),chronic rejection (1 case) and hepatitis recur- rence (1 case),liver re-transplantation was performed on 6 patients through modified piggyback liver transplantation.Vascular anastomosis was performed between the hepatic arteries of donor livers and receptor abdominal aorta in 1 case,and the rest were subjected to end-to-end homonymic vascular anastomosis.Results No operative death occurred.Due to primary non-function,1 died after the ope- ration.During the follow-up period,1 had anastomotic stricture of hepatic artery after the operation and was cured by percutaneous venoplasty.The survival of the rest patients was 12,9,6,4 and 3 months respectively.Conclusion Peritoneal adhesion is the operative difficulty for liver re-transplantation following adult-to-adult living donor liver transplantation.