Expression of BAG3 Gene in Acute Myeloid Leukemia and Its Prognostic Value / 中国实验血液学杂志

<p><b>OBJECTIVE</b>To investigate the expression of BAG3 gene in acue myeloid leukemia (AML) and its prognostic value.</p><p><b>METHODS</b>Real-time quantitative RT-PCR was used to detect the expression of BAG3 mRNA in 88 previously untreated AML patients. The corelation of BAG3 expression level with clinical characteristics and known prognostic markers of AML was analyzed.</p><p><b>RESULTS</b>In 88 patients with AML, the expression of BAG3 mRNA in NPMI mutated AML patients was obviously lower than that in NPMI unmutated patients (P = 0.018). The expression level of BAG3 mRNA did not related to clinical parameters, such as age, sex, FAB subtype, WBC count, extra-modullary presentation, and to prognostic factors including cytogenetics, FLT3-ITD, c-kit and CEBPα mutation status (P > 0.05). The expression level of BAG3 had no obvious effect on complete remission (CR) of patients in first treatment. The expression level of BAG3 in non-M3 patients was higher than that in relapsed patients (P = 0.036). The expression level of BAG3 had no effect on overall survival (OS) of patients.</p><p><b>CONCLUSION</b>The expression level of BAG3 does not correlated with known-prognostic markers of AML, only the expression level of BAG3 in NPM1 mutated patients is lower than that in NPM1 unmutated patients. The expression level of BAG3 has no effect on OS of AML patients, the BAG3 can not be difined as a prognostic marker in AML.</p>

Development and clinical application of fluorescent real-time RT-PCR to hand, foot and mouth disease / 中华微生物学和免疫学杂志

Objective To develop a rapid, accurate, specific method to detect causative agent of hand, foot and mouth disease (HFMD). Methods Specific primers and probe were designed based on highly conserved VP1 region of enterovirus 71, coxsackie virus A16 and enterovirus. The sensitivity and specificity of the real-time RT-PCR was evaluated with 35 stool samples collected from pediatric patients with suspected HFMD and 20 clinical samples from health pediatric patients. Results Out of 35 clinical samples from suspected HFMD, 35 samples were identified as positive for enterovirus, 25 clinical samples were identified as positive for enterovirus 71, 8 clinical samples were identified as positive for coxsackie virus A16, among which 3 clinical samples were identified as positive for enterovirus 71 and coxsackie virus A16. The clinical diagnostic accordance rate is 85.71%. Out of 20 clinical samples from normal pediatric patients, 5 clinical samples were identified as positive for enterovirus, 20 clinical samples were negative for enterovirns 71 and coxsackie virus AI6. Conclusion Our results indicate real-time RT-PCR offers a rapid, sensitive, specific and cheap method to detect pathogen of HFMD from clinical specimens.

HBVcccDNA of patients with hepatitis B in the serum, PBMC and liver tissue distribution / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>Hepatitis B virus covalently closed circular DNA in the serum of patients with hepatitis B, peripheral mononuclear cells (PBMC) and liver tissue distribution.</p><p><b>METHODS</b>Serum HBV DNA > 10(5) copies/ml 50 cases of hepatitis B patients, serum HBV DNA < 10(5) copies/ml 30 cases of hepatitis B patients, patients with fatty liver (hepatitis B) 20 cases, using real-time quantitative polymerase chain reaction for detection of serum, and liver tissue in PBMC HBVcccDNA the existence.</p><p><b>RESULTS</b>Of 50 serum HBV DNA > 10(5) copies/ml cccDNA the serum specimens were 28 cases, 56% detection rate, 29 cases were PBMC HBVcccDNA, 58% detection rate, liver tissue HBVcccDNA were 44 cases, 88% detection rate, serum, the PBMC were detected in liver tissue were significant differences in P < 0.005, compared serum PBMC were no significant differences in P > 0.005. 30 serum HBV DNA < 10(5) copies/ml the serum specimens, PBMC cccDNA detected two cases were 6.67% detection rate, liver tissue cccDNA were six cases 20% detection rate, serum, PBMC, were among the liver tissue was not significantly different P > 0.005. 20 in the serum of patients with fatty liver, liver tissue in PBMCwere not detected HBVcccDNA.</p><p><b>CONCLUSION</b>HBVcccDNA mainly in the liver of patients with hepatitis B, hepatitis B patients and also cccDNA PBMC in the presence of liver tissue but a few of many.</p>

Detection of HBV cccDNA in Peripheral Blood Lymphocyte / 医学研究杂志

Objective Assaying HBV cccDNA in periphral blood lymphocyte and discuss its clinical significance.Methods Using lymphocyte isolating solution to isolate lymphocytes in the peripheral blood, and quantitative florescent realtime PCR was used to detect HBV cccDNA.Results 29 cases of 36 CAH patients was detected positive(80.56%),32 cases of 61 CPH patients was detected positive(52.46%) and 1 case of 59 ASC was detected positive(0.02%) respectively.All 47 healthy people specimen was found negative by the method.CAH group,CPH group and ASC & negative control group show significance statistically(P

Research of Assaying HBV-DNA using PCR Teststrip / 医学研究杂志

10～7/mL specimens,17 were detected highly positive by PCR teststrip method,in 27 cases of 10～5～10～7 specimens 23 were intermediately positive,in 27 cases of 10～3～10～5 specimens 22 were weakly positive,showing statistical significance.All 28 cases

Detection of Chlamydia trachomatis and Ureaplasma urealyticum by Fluorescence Quantitative PCR / 中华医院感染学杂志

OBJECTIVE To discuss the infection rate of Chlamydia trachomatis(Ct) and Ureaplasma urealyticum(Uu) in patients with non-gonococcal infection.METHODS Fluorescence quantitative PCR method was used on 1025 cases and 30 cases of NGU patients for Ct and Uu detection.RESULTS Of 1025 NGU patients,positive Ct alone accounted for 156 cases,the positive rate was 15.22%.505 cases were separate Uu,the positive rate was 49.27%.Ct,Uu mixed in 217 cases,the positive rate was 21.17%.The detection rate was 85.66%.Uu infection rate in women was more than that in men(?2 = 104.56 P0.05).of control group,the Ct Uu Results negative.CONCLUSIONS In NGH patients,Uu is most common pathgen in man and woman.To diagnosis of NGU,Uu and Ct should be followed by Ct infection rate but no gender tested at the same time to avoid missed diagnosis.

Association of histological liver damage with genotypes of HBV DNA in chronic hepatitis B patients in Zhejiang / 中华传染病杂志

0.05). Genotype B+C(2.93?0.74 and 2.95?0.71) was associated with higher grade of necroinflammation and stage of fibrosis than genotype B(1.76?1.18 and 1.76?1.15 ) and C (1.93?1.32 and 1.84?1.29) HBV. Conclusions[KG1]genotype C or B+C HBV are associated with more severe liver damage in patients. Stratification for HBV genotypes should be considered in the antiviral treatment of chronic hepatitis B.