Effect of glycogen on calcium of donor liver during ischemia-reperfusion period / 中华肝脏病杂志

<p><b>OBJECTIVE</b>To evaluate the effect of glycogen on calcium concentration of rabbit donor liver during ischemia-reperfusion period.</p><p><b>METHODS</b>Donor group (n=21) was divided into 3 subgroups randomly: Group A (n=7): fasting for 24 hours before harvesting; Group B (n=7): normal laboratory chew; Group C (n=7): normal laboratory chew plus glucose supplement intravenously. Based on the self-created animal model for ischemia-reperfusion, the levels of glycogen content, ATP level, viability of Ca(2+)ATPase and plasmic free Ca(2+) concentration ([Ca(2+)]i) of liver tissue were measured.</p><p><b>RESULTS</b>Before cold preservation, there was a significant difference of glycogen content among the three groups at all time points except at the end of rewarming period. ATP level and Ca(2+)ATPase viability were significantly higher in group C than in other two groups. But the plasmic free Ca(2+) concentration was lower in groups with higher glycogen content.</p><p><b>CONCLUSIONS</b>Donor liver with high glycogen content can provide relatively sufficient ATP, maintain better Ca(2+)ATPase viability and prevent plasmic free Ca(2+) concentration overloading. This maybe an important mechanism for glycogen to ameliorate ischemia-reperfusion injury to the donor livers.</p>

Protective mechanism of adenosine on intestinal barrier function in acute hemorrhagic necrotizing pancreatitis pigs / 中国普通外科杂志

Objective To study the protective mechanism of adenosine on intestinal barrier function in acute hemorragic necrotizing pancreatitis (AHNP) pigs; Methods Twelve small pigs were equally devided into two groups randomly after the AHNP model sitted up: (1) AHNP controll group (group A) and adenosine treated group (group B). The intestinal blood flow, intestinal permeability, bacterial culture and endotoxin in portal blood were compared between the two groups in pre and post AHNP. Results (1)The intestinal blood flow was dramatically decreased in group A, and much higher in group B than that in group A at the 8 h, 24 hour and day 7 after ANHP model was setted up (P0.001,P

Quantum-Chemical Study of Catalytic Mechanism of Serine Proteases (III) / 昆明医科大学学报

In this paper, the model system of proton transfer with the water molecule as an intermediate acceptor of Ser-195 was suggested and analysed by the CNDO/2 method. The acylation activation barrier of this system was shown to restrict the stage of synchronous transfer of the Ser-195 alcoholic proton and the water molecule proton hydrogen bound to His-57 N~(?_2)-atom to the water molecule oxygen atom and the N~(?_2)-atom, respectively. The substrate protonation in the case of the model system with the water molecule as the in ermediate acceptor was demonstrated to begin before the completion of the tetrahedral inermediate substance, only the protonated form of the tetrahedal intermediate being shown. A lypothesis of considering the role of this water molecule as a nuclephilic reagent in the leacylation stage is presented.

Study on re-warm ischemia-reperfusion injury of the cold-preserved livers and its mechanism / 中国病理生理杂志

AIM: To explore the value of donor liver re-warm ischemia-reperfusion injury during orthotopic liver transplantation and its mechanism. METHODS: The effect of rabbit liver re-warm ischemia on its reperfused liver function and change of malondialdehyde(MDA) and superoxide dismutase(SOD) in the hepatic tissue were observed with rabbit liver preservation-reperfusion injury model . RESULTS: In every group of donor livers, which had experienced re-warm ischemia for 0, 15, 30 and 60 minutes respectively following 6-hours cold storage,the more prolonged re-warm ischemia was, the higher the contents of blood AST, ALT and AKP of receptor rabbits would be, and among all the groups there was significant difference in the contents of AST or ALT or AKP(P

Ouantum Chemical Study on Catalytic Mechanism of Serine Proteases / 昆明医科大学学报

Using the semiempirical MNDO method,several systems simulating the active site of serine proteases have been studied.The stabilization energy was found depending strongly on the nucleophilicity of the attacking group.the decrease of the activation energy has been esti- mated at 9 kca/mole.It was shown that the substrate distortion did not vary with the form- ing of hydrogen bonds.

Quantitative Relation Between Strain and Geometric Paramenter in Cyclic Hydrocarbons / 昆明医科大学学报

In this paper, the quantitative relation between strain and geometirc parameter in cycloalkene, bicylo (n.l.O) alkane and bicyclo (n.m.1) alkane discussed. We found ten excellent linear correlative equations.

CLINICAL USE OF TRANSNASAL ENDOSCOPICAL BILIARY CATHETERIZATION / 解放军医学杂志

Transnasal endoscopical biliary catheterization was done successfully in 44 cases,23 cases with complete data were reported.These cases consisted of decompression and drainage of benign and malignant biliary obstruction (12 cases),dissolution and irrigation treatment of remnants of ascaris in the biliary tract (5 cases),dissolution of gallstone (2 cases),irrigation for biliary calculi (2 cases),hemos-tasis after endoscopic sphincterotomy (EST,1 case),and the examination and culture of the bile ( 1 case).The results were satisfactory without complication.Problems concerning EST was discussed,and endoscopic retrograde biliary drainage (ERBD) and percutaneous transhepatic biliary drainage (PTBD) were compared.

CONTENTS OF MBP AND ET1 IN CSF AND ULTRASTRUCTURAL ALTERATIONS IN LIMBIC SYSTEM IN THE EARLY PERIOD OF STRESS DISORDER FOLLOWING SEVERE INJURY TO DOGS / 解放军医学杂志

By inflicting the hindlimbs of dogs with steel bullets in different velocities, a model of traumatic stress was reproduced. The contents of myelin basic protein (MBP) and endothelin 1 (ET1) in cerebrospinal fluid (CSF) were determined with ELISA and radioimmunoassay methods, and the wltrastructural changes in limbic system were studied with neuropathological techniques. The MBP level and ET1 contents increased markedly in CSF, and neuronal degeneration and nerve fiber demyelination were seen in the hypothalamus and hippocampus in high speed missile group. The results indicate that the hypothalamus and hippocampus were vulnerable in severe injury of remote tissues,and it might be one of the important neuropathological basis for changes in the early stage of posttraumatic stress disorder (PTSD). The results also suggested that the CSF MBP and CSF ET1 determinations might serve as sensitive indicators for central nervous system damage in stress disorder following severe injury.

REMOVAL OF RESIDUAL STONES IN HEPATIC DUCTS THROUGH THE AFFERENT LOOP OF ANASTOMOSIS BETWEEN BILIARY DUCT AND JEJUNUM / 解放军医学杂志

A postoperative follow-up study, through the subcutaneouslyplaced afferent loop of a choledocho-jejunostomy by retrograde cholangiography and/or choledochoscopy in 116 patients with intrahepatic lithiasis, was made. The retained stones, worms, ductal strictures, or suppurative cholangitis were found in 41 cases over a period of one month to six years postoperatively. All these conditions required further management and some even emergency intervention. The patients were all treated successfully by a Dormia basket or other instruments through this conduit with exception of four failures, the overall success rate was 90%. It is suggested that this type of Roux-en-y choledocho-jejunostomy is recommended in the cases with intrahepatic lithiasis which can provide a permanent access from outside to thebiliary tree and appropriate instruments can be passed through this route for diagnostic and therapeutic interventions such as postoperative cholangiography, retained stones removal, and biliary drainage. We believe this route has some advantages over the T-tube tract for non- operative management of intrahepatic residual lesions.