Résumé
A simple, reliable, rapid, precise, sensitive, rugged and validated RP-HPLC method has been developed to determine Metoprolol succinate and Lacidipine in synthetic mixture form. Chromatographic separation achieved isocratically on Prontosil C18 column [5micro m, 250mm × 4.60mm] and acetonitrile: 20mM Phosphate buffer [pH 3.6] in the ratio of 60:40 [v/v] as the mobile phase, at a flow rate of 1 mL/min. Detection was carried out at 278 nm. The mean retention times for metoprolol succinate and lacidipine was found to be 5.25+/-0.5 and 6.72+/-0.5 min, respectively. No interference was found by the excipients in the synthetic mixture. Linearity for metoprolol succinate and lacidipine was in the range of 5-25micro g/mL and 4-20micro g/mL, respectively. The mean recoveries obtained for metoprolol succinate and lacidipine were 100.03 and 99.67 % respectively and RSD was less than 2. The correlation coefficients for all components are close to 1. The proposed method was validated in terms of linearity, range, accuracy, precision, specificity, robustness and the method is successfully applies to the estimation of metoprolol succinate and lacidipine in bulk and in a synthetic mixture
Résumé
Ebola virus disease is one of the most deadly ailments known to mankind due to its high mortality rate (up to 90%) accompanying with the disease. Ebola haemorrhagic fever (EHF) is an infectious disease of animal that can be transmitted to both human and non-human primates. The first epidemic of EHF occurred in 1976 in the Democratic Republic of the Congo. The incubation period of ebola is less than 21 days. Ebola virus infections are depicted by immune suppression and a systemic inflammatory response that leads to damage of the vascular, coagulation and immune systems, causing multi-organ failure and shock. Five genetically distinct members of the Filoviridae family responsible for EHF are as follows: Zaire ebolavirus, Sudan ebolavirus, Côte d'Ivoire ebolavirus, Bundibugyo ebolavirus and Reston ebolavirus. The ongoing 2014 West Africa ebola epidemic has been considered as the most serious panic in the medical field with respect to both the number of human cases and death toll. The natural host for ebola virus is unknown, thus it is not possible to carry out programs to regulate or abolish virus from transmission to people. The ebola virus infection provides little chance to develop acquired immunity causing rapid progression of the disease. It is pertinent to mention that at present, there is no antiviral therapy or vaccine that is helpful against ebola virus infection in humans. The impediment of EHF necessitates much better understanding of the epidemiology of the disease, particularly the role of wildlife, as well as bats, in the spread of ebola virus to humans.
Résumé
A novel, eco friendly, accurate, sensitive, economic and safe spectrophotometric method was developed by application of mixed hydrotropy using 2 M sodium acetate, 8 M urea, 2 M niacinamide and 2 M sodium benzoate solution [25:25:25:25% V/V] as hydrotropic agent, for the solubalizing of poorly water-soluble Furazolidone [FZ] [solubility:- 3.64e-01 mg/mL in water]. There were more than 32 times enhancements in the solubility of FZ were found in mixed hydrotropic solution as compared to solubilities in distilled water. FZ shows maximum absorbance at 360 nm where sodium acetate, urea, niacinamide, sodium benzoate and other tablets excipients did not show any absorbance above 300 nm, and thus no interference in the estimation was seen. FZ was obeyed Beer's law in the concentration range of 10 to 50 micro g/ml r[2]0.9992] in mixed hydrotropic solvent with mean recovery ranging from 97.32% to 98.9%. Proposed method is new, simple, economic, safe, rapid, accurate and reproducible and was validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values