Modulating effects of RAMPs on signaling profiles of the glucagon receptor family

Receptor activity-modulating proteins (RAMPs) are accessory molecules that form complexes with specific G protein-coupled receptors (GPCRs) and modulate their functions. It is established that RAMP interacts with the glucagon receptor family of GPCRs but the underlying mechanism is poorly understood. In this study, we used a bioluminescence resonance energy transfer (BRET) approach to comprehensively investigate such interactions. In conjunction with cAMP accumulation, Gα q activation and β-arrestin1/2 recruitment assays, we not only verified the GPCR-RAMP pairs previously reported, but also identified new patterns of GPCR-RAMP interaction. While RAMP1 was able to modify the three signaling events elicited by both glucagon receptor (GCGR) and glucagon-like peptide-1 receptor (GLP-1R), and RAMP2 mainly affected β-arrestin1/2 recruitment by GCGR, GLP-1R and glucagon-like peptide-2 receptor, RAMP3 showed a widespread negative impact on all the family members except for growth hormone-releasing hormone receptor covering the three pathways. Our results suggest that RAMP modulates both G protein dependent and independent signal transduction among the glucagon receptor family members in a receptor-specific manner. Mapping such interactions provides new insights into the role of RAMP in ligand recognition and receptor activation.

The mediating effect of rumination between social anxiety and mobile phone addiction tendency among college students / 中华行为医学与脑科学杂志

Objective:To explore the mediating effect of rumination between social anxiety and mobile phone addiction tendency among college students.Methods:The social avoidance and distress scale (SAD), mobile phone addiction tendency scale (MPATS) and ruminative response scale (RRS) were used to measure 682 college students.SPSS 25.0 and Mplus 7.0 were used for data analysis.Pearson correlation analysis was used to explore the correlation among social anxiety, mobile phone addiction tendency and rumination.Bootstrap method was used to test the mediating effect of rumination between social anxiety and mobile phone addiction tendency among college students.Results:The total score of social anxiety was (13.36±6.02), the total score of rumination was (51.04±11.56), and the total score of mobile phone addiction tendency was (40.46±11.74). The detection rate of mobile phone addiction tendency was 23.90%, and the detection rate of social anxiety was 49.85%.The total score of social anxiety was positively correlated with the total score of rumination ( r=0.31, P<0.01) and the total score of mobile phone addiction tendency ( r=0.25, P<0.01). The total score of rumination was positively correlated with the total score of mobile phone addiction tendency ( r=0.46, P<0.01). The structural equation model showed that rumination played a complete mediating role between high social anxiety and mobile phone addiction tendency, and the effect value was 0.18.There was no mediating effect between low social anxiety and mobile phone addiction tendency, and the direct effect value was 0.33. Conclusion:Low social anxiety directly affects college students' tendency of mobile phone addiction, while high social anxiety indirectly affects college students' tendency of mobile phone addiction through rumination.

Comparative analysis of clinical characteristics and prognosis between bacterial necrotizing pneumonia and Mycoplasma pneumoniae necrotizing pneumonia in children / 中华儿科杂志

Objective@#To compare the characteristics of Mycoplasma pneumoniae necrotizing pneumonia (MPNP) and bacterial necrotizing pneumonia (BNP), and explore the biomarkers for differentiation of MPNP from BNP.@*Methods@#A retrospective, observational study of 52 necrotizing pneumonia (NP) cases who were hospitalized in our hospital from January 2008 to December 2017 was conducted. According to the pathogen causing NP, patients were divided into two groups, BNP and MPNP, and the clinical manifestations, laboratory data, imaging findings, hospital course and prognosis between these groups were analyzed.@*Results@#This study enrolled 19 boys and 33 girls, and the median ages of patients were 4.4 (0.1-13.8) years old. Of the totally of 52 NP patients, 19 were in the BNP group (9 boys and 10 girls), 33 were in the MPNP group (10 boys and 23 girls). The mean age of MPNP patients was much older than that of BNP patients (5.2 (2.3-13.2) years vs. 1.8 (0.1-13.8) years, Z=-0.128, P<0.01). The number of patients with tachypnea and pleural effusion septation were significantly higher in BNP patients than those in MPNP patients (15 cases vs. 4 cases, χ2=23.222, P<0.01; 14 cases vs. 1 case, χ2=29.326, P<0.01), which more needed to oxygentherapy (18 cases vs. 12 cases, χ2=16.833, P<0.01) and undergo chest drainage (9 cases vs. 4 cases, χ2=5.829, P=0.022); while the number of patients required bronchoalveolar lavage was higher in MPNP patients than that in BNP patients (5 cases vs. 32 cases, χ2=29.326, P<0.01). The values of white blood cell (WBC) (23.2 (5.2-67.1)×109/L vs. 9.7 (6.3-18.7)×109/L, Z=-4.855, P<0.01), procalcitonin (PCT) (3.69 (0.23-90.15) mg/L vs. 0.28 (0.02-1.44) mg/L, Z=-3.207, P=0.001), C reactive protein (CRP) (160 (94-220) mg/L vs. 90 (5-134) mg/L, Z=-4.337, P<0.01), interleukin (IL)-10 (11.7 (4.2-401.5) ng/L vs. 4.8 (2.0-23.4) ng/L, Z=-2.278, P=0.023), pleural fluid cell count (5 200 (120-50 000)×106/L vs. 790 (68-6 920)×106/L, Z=-3.125, P=0.002), pleural fluid lactic dehydrogenase (LDH) (3 990 (589-29 382) U/L vs. 2 211 (673-3 993) U/L, Z=-2.488, P=0.013) in BNP group were significantly higher than those in MPNP group; while the values of pleural fluid glucose(0.43 (0.03-18.00) mmol/L vs. 5.95 (4.27-7.87) mmol/L, Z=-2.795, P=0.005), serum tumor necrosis factor (TNF)-α (2.3 (1.0-2.8) ng/L vs. 2.6 (1.3-109.2) ng/L, Z=-2.113, P=0.035) and interferon (IFN)-γ (4.8 (2.6-7.7) ng/L vs. 11.9 (2.9-154.6) ng/L, Z=-2.455, P=0.014) were lower in BNP group than those in MPNP group. Meanwhile, the mean time from the onset of symptoms to the discovery of necrotic lesions was longer in MPNP group than that in BNP group ((20.6±6.4) days vs. (14.6±6.2) days, t=3.029, P=0.004). After treatments, all patients were discharged without death, WBC and PCT recovered more quickly in MPNP group than those in BNP group (12 (0-24) days vs. 0 (0-23) days, Z=-4.484, P<0.01; 10 (5-15) days vs. 0 (0-23) days, Z=-3.244, P=0.001). As to prognosis, 34 cases were followed up, and the results showed that patients recovered without surgical intervention, and chest lesions were resolved within 3.0 (1.0-8.0) months, and the time to necrosis disappearance was similar in the BNP group and MPNP greup (3.0 (1.0-8.0) months vs. 3.0 (1.0-8.0) months, Z=-0.128, P=0.001). In receiver operator characteristic curve analysis, the cut-off values for the age, WBC, CRP, PCT, pleural fluid cell count and pleural fluid glucose were set at 2.4 years of age, 17.2×109/L, 157 mg/L, 1.505 mg/L, 2 630×106/L and 3.73 mmol/L, respectively.@*Conclusions@#NP is found to be severe and prolonged, yet, reversible through proper therapy, such as rational antibiotics application. The age, WBC, CRP, PCT, pleural fluid cell count and pleural fluid glucose could be used as biomarkers to differentiate MPNP from BNP in children.

Energy supply nutrients and mineral composition analysis in breast milk in Lanzhou urban / 中华临床营养杂志

Objective To analyze the energy supply nutrients (protein,fat and lactose) and mineral elements (calcium,copper,iron,zinc,magnesium,sodium,potassium) supplied in breast milk from the resident of Lanzhou,and investigate the problems of those energy supply nutrients and mineral elements in breast milk of this population so that to provide basis for breast feeding.Methods Breast milk from 1 215 mothers whose babies were fed totally by the breast milk and grew well was collected and assayed for the contents of protein,fat,lactose,calcium,copper,iron,zinc,magnesium,sodium and potassium in Lanzhou region.The optimal range for breast feeding was set by normal distribution method.The results were compared with the breast milk from other internal region in order to clarify the contents difference.Results The prevalence of mother-milk-fed in this region was 48.5％.The normal ranges (unit:g/100 g) of fat,lactose and protein were 1.57-5.11,6.58-7.16 and 0.79-1.35,and the normal ranges of calcium,copper,iron,zinc,magnesium,sodium and potassium were 5.25-7.83 mmol/L,5.66-8.28 mmol/L,0.14-0.3 mmol/L,15-64.48 μmol/L,0.98-1.62 mmol/L,1.92-18.82 mmol/L,7.95～ 17.31 mmol/L in the high quality breast milk.Compared with the other internal region,the normal ranges of the protein,fat and lactose in Lanzhou were lower.The contents of protein,fat,lactose,calories,calcium,copper,zinc and sodium in the breast milk from the whole investigated population were significantly lower compared with breast milk of high quality (P＜0.01).Conclusion The quality of breast milk in Lanzhou is not so good.The contents of various nutrients in breast milk should be improved to a proper level by appropriate ways so that to improve the growth and development of the baby.

Orthogonal assays for the identification of inhibitors of the single-stranded nucleic acid binding protein YB-1

We have previously shown that high expression of the nucleic acid binding factor YB-1 is strongly associated with poor prognosis in a variety of cancer types. The 3-dimensional protein structure of YB-1 has yet to be determined and its role in transcriptional regulation remains elusive. Drug targeting of transcription factors is often thought to be difficult and there are very few published high-throughput screening approaches. YB-1 predominantly binds to single-stranded nucleic acids, adding further difficulty to drug discovery. Therefore, we have developed two novel screening assays to detect compounds that interfere with the transcriptional activation properties of YB-1, both of which may be generalizable to screen for inhibitors of other nucleic acid binding molecules. The first approach is a cell-based luciferase reporter gene assay that measures the level of activation of a fragment of the promoter by YB-1. The second approach is a novel application of the AlphaScreen system, to detect interference of YB-1 interaction with a single-stranded DNA binding site. These complementary assays examine YB-1 binding to two discrete nucleic acid sequences using two different luminescent signal outputs and were employed sequentially to screen 7360 small molecule compounds leading to the identification of three putative YB-1 inhibitors.

The value of MRI in early diagnosis of dysbaric osteonecrosis / 中华劳动卫生职业病杂志

Objective@#To investigate the value of MRI in the early diagnosis of diagnosis of dysbaric osteonecrosis.@*Methods@#Labor hygiene investigation and occupation health were examined on 52 high pressure operating personnel, were selected for the examination of both shoulders, hips and knees with X-ray and CT scan.@*Results@#The cystic sign in dysbaric osteonecrosis as an important imaging feature, which perform in the MRI examination for T1W I sequence showed low or slightly low signal and T2W I sequence showed high signal, and X-ray and CT have a lower detection rate than MRI. The Kappa consistency test showed a high consistency with the two methods. At the same time MRI examination also can discover the bone marrow cavity necrosis early pathological change.@*Conclusion@#MRI is an effective method for the diagnosis of early dysbaric osteonecrosis, which can improve the early diagnosis rate of dysbaric osteonecrosis.

Efficacy of bronchoalveolar lavage and its influence factors in the treatment of Mycoplasma pneumoniae pneumonia with atelectasis / 中华儿科杂志

Objective@#To investigate the efficacy of bronchoalveolar lavage (BAL) and its influence factors in the treatment of Mycoplasma pneumoniae pneumonia (MPP) with atelectasis.@*Methods@#A retrospective case control study was performed on hospitalized MPP patients with atelectasis and received BAL in the Department of Pulmonology, Children's Hospital Zhejiang University School of Medicine from January 1, 2015 to July 31, 2017. Fever relieved in 48 hours and chest imaging improved in one week after BAL were considered effective. Clinical data, including age, sex, blood routine tests, lactate dehydrogenase (LDH), cytokines, complications, fever duration before BAL, course of disease before BAL, sputum plug, atelectasis area and its CT values of atelectasis site were collected. Student's t test, Mann-Whitney U test, or chi square test were used.@*Results@#(1) A total of 163 patients were enrolled, including 69 boys and 94 girls, with the ratio of 1∶1.36. Their ages ranged from 6 months to 12.6 years. (2) On the day of bronchoscope, 113 patients still had fever. They were divided into effective group (n=66) and ineffective group (n=47) according to whether fever was relieved in 48 hours after BAL. The effective group were found to have less sputum plug compared with the ineffective group (33% (22/66) vs. 57% (27/47), χ2=6.499, P=0.011). The other factors such as sex, age, fever duration before BAL, course of disease before BAL, C reactive protein (CRP), LDH, IL-2, IL-4, IL-6, IL-10, TNF, IFN-γ, atelectasis area and CT value showed no significant difference between the two groups (all P>0.05). (3)A total of 122 cases had chest imaging after BAL. According to chest imaging improvement, they were divided into effective group (n=81) and ineffective group (n=41). The effective group showed lower CT value ((58±9) vs. (63±8) HU, t=-2.436, P=0.017), IL-6 and IL-10 (M(Q1, Q3)) (21.0 (1.9, 48.4) vs. 36.4(21.8, 93.6), 4.9 (3.7, 9.6) vs. 7.7 (4.4, 12.0) ng/L, Z=-2.387,-2.009, P=0.017, 0.045). Sex, age, fever duration before BAL, course of disease before BAL, CRP, LDH, IL-2, IL-4, TNF, IFN-γ, atelectasis area showed no significant differences between the two groups (all P>0.05). (4) Patients were divided into sputum plug group (57 cases) and non sputum plug group (106 cases) according to bronchoscopic findings. The sputum plug group showed higher LDH, CRP, IL-6, IFN-γ, incidence of pleural effusion and extrapulmonary complications (585(433, 833) vs. 369 (312, 588) U/L, 42 (19, 103) vs. 25 (12, 45) mg/L, 38.8 (22.1, 71.3) vs. 20.7 (9.2, 48.3) ng/L, 33.1 (13.5, 89.3) vs. 12.7 (6.5, 33.6) ng/L, 73.7% (42/57) vs. 52.8% (56/106), 40.4% (23/57) vs. 17.0% (18/106)), with statistically significant differences (Z=-4.865,-3.435,-3.098,-3.704, χ2= 0.010, 0.001, all P<0.01) . Additionally, fewer patients showed fever relief within 48 hours after BAL in the cases with sputum plug cases compared those without sputum plug (44.9% (22/49) vs. 68.8% (44/64), χ2= 0.011, P=0.009). Fewer patients showed chest imaging improvement within one week after BAL in the cases with sputum plug compared with those without sputum plug, but did not show significant difference (56.5% (26/46) vs. 72.4% (55/76), χ2=0.073, P=0.056).@*Conclusions@#BAL has some therapeutic effect on fever or atelectasis in MPP children complicated with atelectasis. Chest imaging improvement or fever relief may be hampered by sputum plug, increased IL-6 or IL-10.

High-throughput screening for small molecule inhibitors of the type-I interferon signaling pathway

Interferons (IFNs) are cytokines with fundamental roles in resistance to infections, cancer and other diseases. Type-I IFNs, interferon (IFN-) and interferon (IFN-), act through a shared receptor complex (IFNAR) comprised of IFNAR1 and IFNAR2 subunits. Binding of type-I IFN to IFNAR1 will robustly activate Janus activated kinase-signal transducer and activator of transcription (JAK-STAT) signaling pathway. Aberrant activation of the type-I IFN response results in a spectrum of disorders called interferonopathies. The purpose of this research is to develop an assay for high-throughput screening (HTS) of small molecule inhibitors of the type-I IFN signaling pathway. Inhibition of type-I IFN signaling can be beneficial in terms of therapeutic use and understanding the underlying mechanism of action. We report here a HTS campaign with the secreted embryonic alkaline phosphatase (SEAP) reporter gene assay against 32,000 compounds which yielded 25 confirmed hits. These compounds were subsequently characterized for their cytotoxicity, effects on STAT phosphorylation and activities in IFN regulatory factor (IRF) transcription.

Effectiveness of methylprednisolone in treatment of children with refractory Mycoplasma pneumoniae pneumonia and its relationship with bronchoalveolar lavage cytokine levels / 中华儿科杂志

<p><b>OBJECTIVE</b>To investigate cytokine level in bronchoalveolar lavage fluid (BALF) in children with refractory Mycoplasma pneumoniae pneumonia (RMPP) and the effects of methylprednisolone on RMPP.</p><p><b>METHOD</b>Sixty cases with RMPP and 20 cases with bronchial foreign body with no respiratory tract infection as control group hospitalized in Department of Pulmonary Diseases, the Children's Hospital Affiliated to Medical School of Zhejiang University from February 2012 to February 2013 were enrolled. The RMPP patients were divided into two groups randomly (30 cases in each). Steroid group were given methylprednisolone 2 mg/(kg·d) intravenously for 3 days, and the cases in non steroid group were not given steroid therapy. Patients whose fever relieved after steroid treatment were classified as defervesced group while the others were classified as non defervesced group. Each patient was examined with fiberoptic bronchoscopy and bronchoalveolar lavage 3 days after admission and cytokine level in BALF of each patient was detected.</p><p><b>RESULT</b>(1) In steroid group, the proportion of patients whose fever disappeared within 3 days after steroid therapy was 9/30 cases (30%), and in non steroid group no one responded within 3 days after medication, showing statistically significant difference (χ² = 14.073, P=0.002), at the same time, the duration of cough in steroid group was significantly shorter than that in non steroid group (5.1 d vs. 7.0 d, t=-2.276, P=0.027). The total fever time of steroid group was 4.7 days, which as compaired with non steroid group (6.7 days) was shorter, but the difference was not significant (t=-1.351, P=0.134). (2) IL-1 β, IL-4, IL-6, IL-8, IL-10, IFN-γ in BALF of steroid group and non steroid group were both significantly higher than that of control group. But the same comparison between steroid group and non steroid group showed no significant difference. (3) In steroid group, IL-2 and IL-8 in BALF of patient whose fever disappeared after steroid therapy were both significantly lower than that of patients who still had fever (t=2.771, 2.054, P=0.010, 0.049) , but no significant difference was found between the two groups in BALF IL-1 β, IL-4, IL-6, IL-10, IFN-γ levels (P>0.05).</p><p><b>CONCLUSION</b>(1) Three days of 2 mg/(kg·d) methylprednisolone therapy had the antipyretic effect in children with RMPP, and could shorten the length of cough. (2) Incresed BALF IL-1 β, IL-4, IL-6, IL-8, IL-10, IFN-γ levels were observed in RMPP and high level of BALF IL-2 and IL-8 might have some relevance with persistent fever of RMPP in children.</p>

Aorta diseases misdiagnosed as simple inferior limb thrombo-embolism in 9 cases / 中华普通外科杂志

Objective To analyze the causes of misdiagnosis of aorta diseases for simple inferior limb artery thrombo-embolism,and summarize the clinical experience. Methods Retrospective analysis was made on clinical data of 9 eases misdiagnosed aorta disease,including clinical manifestation,misdiagnosis,improper treatment and final definite diagnosis. Results All 9 cases were misdiagnosed as simple inferior limb thrombo-embolism at first.Three cases were treated with emergent thrombectomy using Fogarty catheter.The correct diagnosis Was achieved by 3-dimensional CT angiography (3DCTA) after operation,and the eitiology of other 6 cases were also pmved as aortic disease by 3DCTA before operation.Among 5 cases of acute aorta dissection with iliac-femoral artery involved,2 cases abandoned surgery with one dying the next day and the other lost to follow-up after being discharged.The other 3 cases were treated with endovascular therapy successfully.One case of abdominal aorta anurysm with mural thrombosis defluxion were treated by aneurysm resection.The other 3 caBes of Leriche syndrome with acute aorta terminal filament thrombosis formation were cured by aortoiliac bypass.The limbs ischemia were improved in all cases without perioperative death.Conclusion Aorta diseases can sometimes lead to acute inferior limb ischemia,mimicking limb artery thrombo-embolism.Preoperative imaging especially 3 DCTA helps to establish correct preoperative diagnosis for a successful treatment.

Depression, Anxiety and Self-concept of Middle School Students / 中国心理卫生杂志

Objective:to study depression, anxiety and self-concept of middle school students.Method:Self-description Questionnaire, CES-D and Revised Children's Manifest Anxiety Scale were used to assess 500 middle school students of Nanchong, Sichuan province.Result:the total score of self-concept was negatively correlated with anxiety and depression. Factors predicting anxiety and depression included general self-concept, general expressive self-concept, parenthood self-concept and peer relation self-concept.Conclusion:anxiety and depression of middle school students are associated with their self-concept.

Anxiety of Middle School Students——The Role of Teacher-Student Relationship and Peer Relationship / 中国心理卫生杂志

Objective: To study the role of teacher-student relationship and peer relationship in anxiety of middle school students. Method: Revised Children'sManifest Anxiety Scale, Teacher-Student Relationship Questionnaire and Scale for Assessing Peer were used to investigate anxiety problems in 500 students grade 1 to 3 of three middle schools in Nanchong, Sichuan. Result: Anxiety had close correlation with teacher-student relationship and with peer relationship. Better teacher-student relationship or peer relationship contributed to relax anxiety of students. Further regression analysis revealed anxiety was predictable by positive and negative emotions. Conclusion: This result provides a base for further study on anxiety of middle school students.

The effect of p27~(kip1) gene transfection mediated by PLGA nanoparticles on neointimal proliferation and apoptosis in rats vein grafts / 中国普通外科杂志

Objective To investigate whether human p27kip1 gene transfection mediated by nanoparticles( NP) can express p27kip1 protein and inhibit the neointimal formation in rat vein grafting models. Method Autogenous vein graft model was established in 120 Wistar rats by transplanting internal branch of jugular vein to carotid artery. Rats were divided into three groups: (1) p27 group; p27kip1 gene mediated by NP were transfected into the veins before anastomosis; (2) control group; the veins were transfected by simple NP; (3) grafting group;the veins were grafted without transfection. The grafted veins were harvested at 3 d, 7 d, 14 d and 28 d respectively after the operation. The exogenous p27 kip1 protein expression in veins was determined by Western blot. Intimal hyperplasia (IH) and PCNA were observed by pathology and analyzed by computer digitizing system. The presence of apoptotic VSMC was demonstrated by TUNEL method. Result p27kip1 gene transfection mediated by NP complex increased protein expression of p27kip1 gene and there was a significant decrease in the intimal average thickness at 7 d, 14 d and 28 d in p27 group (P

Experimental study of Egr-1,PDGF-B and TGF-?_1 genes in autogenous vein graft / 中国普通外科杂志

Objective To investigate the expression and relationship of early growth response gene-1((Egr-1)),platelet-derived growth factor-B(PDGF-B) and tranforming growth factor(TGF-?_1) in autogenous vein graft in rats,and the role in vein graft intimal hyperplasia(IH).Methods Autogenous vein graft model was(established) in 90 wistar rats.The vein graft samples were harvested at 1,2,6,24 hours,and 3,7,14,28,42 days after surgery.Normal vein was used as control group.Egr-1、PDGF-B,TGF-?_1 mRNA was measured by reverse transcription-PCR and in situ hybridization.Western blotting and immunohistochemistry were used to detect the protein expression of Egr-1,PDGF-B and TGF-?_1. Results Expression of Egr-1,PDGF-B,TGF-?_1 mRNA and protein was not detected in normal vein.In grafting vein,expression level of Egr1mRNA reached a peak at 28days,and the positive rate of Egr-1mRNA was 45%?6%;(PDGF-BmRNA) reached a peak at 14days(48%?6%);a peak of TGF-?_1mRNA was 46%?9% reached at 7days;Egr-1 protein expression reached a peak at 28days, and the positive rate of Egr-1 protein was 40%?9%.PDGF-B protein reached a peak at 28days(45%?4%),TGF-?_1 protein reached a peak at 14days(41%?7%).Conclusions Intimal hyperplasia of vein graft is closely associated withexpression of Egr-1、PDGF-B and TGF-?_1;the activation and expression of PDGF-B and TGF-?_1 may be(modulated) by Egr-1,and they may contribute to increase expression of Egr-1 by feedback.

Treatment of acute abdominal aorta saddle embolism:a report of 21 cases / 中国普通外科杂志

Objective To summarize our experience in treating abdominal aorta saddle embolism(ASE).Methods The clinical data of 21 cases of abdominal ASE were treated with Fogarty catheter and other(methods) during January 2000 to July 2006 were retrospectively assessed.Results After the blood flow was restored by operation,4 died in the postoperative early stage because of sudden cardiac asystole due to(hyperkalemia);in the late stage,6 died of multiple organ dysfunction syndrome socondary from acute renal(failure)(ARF).Eleven patients were cured.Of them,bilateral lower extremites were salvaged in 5 patients;and 6 patients received amputation.Ten patients were followed up,and the blood supply of the salvaged legs was good.Conclusions Early diagnosis and embotism removal are the key points to decrease the mortality and amputation rate of ASE.The intra-operative and post-operative prevention and management of(hyperkalemia) and ARF are important for reduction of mortality.

The effect of antisense mTOR gene mediated by nanoparticles on neointimal formation in vein graft in rats / 中国普通外科杂志

Objective To study the effect of antisense mTOR gene transfection mediated by nanoparticles(NP) on intimal perliferation after vein grafting.Methods Nanoparticle antisense mTOR gene complex was prepared with PLGA and PVA.Autogenous vein graft model was established in 72 rats by transplanting internal branch of jugular vein to carotid artery.Three groups were studied:(1) antisense mTOR group,antisense mTOR gene mediated by NP was transfected into the veins before anastomosis.(2) Empty vector group,the vein was transfected by empty vector mediated by NP.(3) Control group,no transfection.The grafted veins were harvested 3 days,1 week,2 weeks and 4 weeks after operation,respectively.The exogenous mTOR mRNA and protein expression were determined and intimal hyperplasia(IH) was observed.The presence of apoptotic VSMC was also detected.Results Antisense mTOR gene transfection mediated by nanoparticle complex inhibited the mRNA and protein expression of mTOR gene(P

Expression and significance of p38 mitogen-activated protein kinases in autogenous vein graft / 中国普通外科杂志

ObjectiveTo investigate the expression and significance of p38 mitogen-activated protei n kinase (MAPK) in autogenous vein graft. Methods Autogenous vein graft model was established by transplanting the right jugular vein to infr arenal abdominal aorta in 80 Wistar rats. Ten vein graft samples were harvested 6 hours, 24 hours, 3 days, 7 days, 2 weeks, 4 weeks, 6 weeks and 8 weeks after s urgery,respectively. Reverse transcription-PCR and in situ hybridization,Wester n blot and immunohistochemistry methods were used to detect the expression of pr otein and phosphorylation protein of p38 and p38mRNA. ResultsThe expression of p38 mRNA increased 6 hours after surgery and reached the peak on the second week after surgery (59%?26%),and significantly higher than that on 4,6,8 weeks( P

Management of myonephropathic-metabolic syndrome after acute arterial occlusion: report of 17 cases / 中国普通外科杂志

ObjectiveTo investigate the management of myonephropathic-metabolic syndrome (MNMS) after acute arterial occlusion. Methods17 cases of MNMS caused by acute arterial occlusion were restrospectively reviewed. Results5 cases were cured, 6 cases died of sudden cardiac arrest induced by hyperkalemia, and another 6 cases died of multiple organ dysfunction syndrome (MODS) complicated by acute renal failure. The total mortality rate was 71% and the amputation rate was 41%.ConclusionEarly revascularization should be performed in acute arterial occlusion. In patients with compartment syndrome, fasciotomy should be performed as soon as possible. Early amputation of gangrene limb is very important to prevent MNMS. Early and effective fluid resuscitation and alkalinization is the key point to prevent ARF, early hemodialysis for ARF is very important in treating MNMS.

The expression of hypoxia-inducible factor-la and related genes in ruptured abdominal aorta aneurysm / 中国普通外科杂志

Objective To study the expression of hypoxia-inducible factor(HIF)-1? and related genes in ruptured abdominal aorta aneurysm(RAAA) and explore the underlying pathogenesis. Methods 18 RAAA specimens were collected and 20 AAA tissues were used as control. Total RNA was extracted and Northern blot was used to evaluate the expression of HIF-1? mRNA. Western blot and immunohistochemistry method were used to determine the expression of HIF-1? and vascular endothelial growth factor(VEGF). Microvessel density(MVD) was measured for CD34. Results The expression of HIF-la mRNA was significantly higher in RAAA than that in AAA (P

The expression and significance of early growth response gene-1 in autogenous vein graft in rats / 中国普通外科杂志

Objective To investigate the expression of early growth response gene-1 in autogenous vein graft in rats, and its role in intimal hyperplasia (IH).Methods Autogenous vein graft model was established in 90 Wistar rats. Grafts were harvested at 1、2、6、24 hours, and 3、7、14、28、42 days after surgery. Normal veins serve as control. Egr-1 mRNA was measured by RT-PCR and in situ hybridization. Western blotting and immunohistochemistry were used to detect the protein expression of Egr-1. Results The expression of Egr-1 mRNA and Egr-1 protein experienced biphasic changes. At 1 hour, the value of gene expression of Egr-1 mRNA was 2. 21?0. 85, declining at 6 and 24 hours, and upgrading at 7 days, culminating at 3.16?1.14 at 28 days. Egr-1 protein expression was detected at 2 hours, with the positive expression rate of 31%?6%. The level of Egr-1 protein was decreased from 24 hours to 3 days. A peak was reached at 41%?8% at 28 days. Immunoreative Egr-1 was located within vascular smooth muscle cells (VSMCs) and monocytes/macrophages in the media at the early phase of day 7 and 14, and in neointimal and medial VSMCs at later phase of day 28. Egr-1 was also present in the endolumial endothelial cells. Conclusion In autogenous vein graft, Egr-1 plays an important role in the proliferation of VSMCs in autogenous vein graft.