Study on the change of bFGF in reticular formation of medulla oblongata after primary brain-stem injury / 法医学杂志

OBJECTIVE@#To study the effect of primary brain-stem injury on the expression of basic fibroblast growth factor (bFGF) in the reticular formation of medulla oblongata.@*METHODS@#Immunohistochemical SABC was used to study the change of bFGF expression in the reticular formation of medulla oblongata after brain-stem injury by striking.@*RESULTS@#The numbers of positive cells and positive intensity of the study group in the reticular formation of medulla oblongata were significantly elevated than those of the control group and the postmortem injury group.@*CONCLUSION@#The expression of bFGF is elevated in reticular formation after brain-stem injury.

Study on the relationship between PMI and the concentration of zincum and nickel in the vitreous humor of rabbit after death / 法医学杂志

OBJECTIVE@#To seek a exact method of estimating postmortem interval (PMI).@*METHODS@#This study detected the concentration of zincum(Zn) and nickel(Ni) in vitreous humor of rabbit at hour 96 after death and explored the relationship between their concentration and PMI using a method ICP-MS.@*RESULTS@#The concentration of Zn and Ni in vitreous humor of rabbit at hour 24 after death were related to PMI significantly; The formulae of the relationship between PMI and Zn concentrations is y = 0.1404x2 - 1.3351x + 3.8298 (within 24 h; R2 = 0.9202). The formula of the relationship between PMI and Ni concentrations is y = 0.0043x2 - 0.0596x + 0.2665(within 24 h; R2 = 0.9103).@*CONCLUSION@#The concentration of Zn and Ni in vitreous humor of rabbit may be a reference indicator to estimate early PMI.

Study on the relationship between postmortem interval and the change of absorbance in vitreous humor of rabbit after death / 法医学杂志

OBJECTIVE@#To seek a exact method of estimating Postmortem interval (PMI).@*METHODS@#This study was preformed to investigate the relationship between postmortem interval and absorbance in vitreous humor of rabbit after death. The absorbance in vitreous humor of 48 rabbits after death were investigated with Model 754 spectrophotometer in apt wavelength (420 nm).@*RESULTS@#There exists positive linear regression association between postmortem interval (Y) and absorbance in vitreous humor (X) (r = 0.98327, P < 0.05), during rabbits after death 0 to 72 hours. The formula of linear regression is Y = 453.30 X + 0.75 (Y = postmortem interval = PMI, X = absorbance in vitreous humor).@*CONCLUSION@#The absorbance in vitreous humor can be as reference indicator to estimate PMI within hour 72.